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Environmental response type glucosyl nanoparticle and processing method thereof

A nanoparticle and environment-responsive technology, which is applied in the direction of pharmaceutical formulations, medical preparations of non-active ingredients, antibody medical ingredients, etc., can solve the problems of few research reports on natural glucosyl nanopolymers, poor safety, and technical difficulty. , to achieve the effect of improving antigen bioavailability and excellent pH stimulation response effect

Active Publication Date: 2020-04-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many nanocarrier material technologies are difficult to achieve commercial production on an industrial scale due to high cost and technical difficulty.
At present, chemically synthesized nanomaterials mainly include polyalkylcyanoacrylates (methyl esters, ethyl esters, butyl esters, etc.) and polyesters (polylactic acid, polylactide, polycaprolactone, etc.) and their derivatives and copolymers. , low biocompatibility and poor safety, there are few research reports on natural glucosyl nanopolymers, and there has been no report on environmental responsive natural glucosyl nanoparticles

Method used

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  • Environmental response type glucosyl nanoparticle and processing method thereof
  • Environmental response type glucosyl nanoparticle and processing method thereof
  • Environmental response type glucosyl nanoparticle and processing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Weigh 1g of glucosyl nanoparticles derived from sweet sorghum (molecular weight: 2.1×10 7 g / mol), dissolved in pH 7.0 sodium acetate buffer solution, made into a homogeneous solution with a mass concentration of 10%, added 80U amyloglucosidase, stirred and reacted at a constant temperature for 5 hours under the condition of 50°C, and the precipitate was dried after the enzyme treatment Treatment: Take the nano-particles after the surface thinning treatment to make a solution with a mass concentration of 20%, add 10% octadecenylsuccinic anhydride, adjust the pH value to 8.5, place it in a microwave field with a power of 600W and keep it at 40°C for reaction 8h, the target product was obtained.

[0029] The obtained environment-responsive glucose-based nanoparticles contain 3.5% of short-chain DP 2-5, have an average particle diameter of 45nm, and a dispersed molecular density of 760g / mol·nm 3 , surface zeta potential -21mV.

[0030] Environmental stimulus response effe...

Embodiment 2

[0032] Weigh 1 g of Arabidopsis-derived glucosyl nanoparticles (molecular weight: 6.3×10 6 g / mol), dissolved in pH 3.5 sodium acetate buffer solution, made into a homogeneous solution with a mass concentration of 5%, added 10U maltosidase, stirred continuously at 30°C for 2 hours, and precipitated and dried after deactivation treatment ; Take the nano-particles after the surface thinning treatment to form a solution with a mass concentration of 5%, add 0.5% maleic anhydride, adjust the pH value to 11, place it in an ultrasonic field with a frequency of 50kHz and keep it at 37°C for 4h, that is Obtain the target product.

[0033] The resulting environment-responsive glucose-based nanoparticles contain 2.1% of short-chain DP 2-5, have an average particle diameter of 32nm, and a dispersed molecular density of 490g / mol·nm 3 , surface zeta potential -35mV.

[0034] Environmental stimulus response effect: when the pH in the environment changes from 3 to 12, the surface potential o...

Embodiment 3

[0036] Weigh 1g of glucosyl nanoparticles derived from red algae (molecular weight: 9.8×10 7 g / mol), dissolved in pH 6.0 sodium acetate buffer solution, made into a homogeneous solution with a mass concentration of 20%, added 200U amylolytic enzyme, stirred and reacted at a constant temperature for 0.5h under the condition of 70°C, and dried the precipitate after the enzyme treatment Treatment: Take the nano-particles after the surface thinning treatment to make a solution with a mass concentration of 50%, add 3% tripolyphosphate, adjust the pH value to 7, place it in a microwave field with a power of 200W and keep it at 45°C for 1.5h. That is, the target product is obtained.

[0037] The obtained environment-responsive glucose-based nanoparticles contained 5.7% of short-chain DP 2-5, the average particle size was 74 nm, and the dispersed molecular density was 620 g / mol·nm. 3 , the surface zeta potential -35mV.

[0038] Environmental stimulus response effect: When the pH in th...

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Abstract

The invention discloses an environmental response type glucosyl nanoparticle and a processing method thereof, and belongs to the technical field of starch-based material processing. The environmentalresponse type nanoparticle is prepared from a glucosyl nanoparticle through a novel enzyme surface thinning-charge positioning modification composite modification technology. The method has the characteristics of simple and controllable process, greenness, environmental protection, continuous production and the like, and the prepared product can be used in the fields of foods, medicines, chemicalindustry and the like as a matrix substance.

Description

technical field [0001] The invention relates to an environment-responsive glucose-based nanoparticle and a processing method thereof, belonging to the technical field of starch-based material processing. Background technique [0002] As a cutting-edge interdisciplinary emerging subject field developed in the past two decades, nanotechnology has the ultimate goal of directly manufacturing products with specific functions based on the novel physical, chemical and biological properties of substances at the nanometer scale, bringing relevant Revolutionary progress in the industry. At present, the application field of nanotechnology not only involves high-tech fields such as electronics, information, and life sciences, but also has gradually penetrated into traditional industries such as chemical industry, machinery, medicine, and food, and has gradually developed into an important strategy in the field of medicine and food. platform. Developed countries such as Europe, the Uni...

Claims

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Application Information

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IPC IPC(8): C08B31/00A61K39/39A61K47/69
CPCC08B31/00A61K47/6939A61K39/39A61K2039/55583
Inventor 缪铭刘瑶陈一枚张涛胡锦华
Owner JIANGNAN UNIV
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