Fluorescence collection and fluorescence separation structure

Abstract

The invention discloses a fluorescence collection and fluorescence separation structure. The structure comprises a pair of first and second optical channels which are symmetrically arranged on two sides of a flow cell, aspheric mirrors are arranged at the inlet ends of the optical channels, and fluorescence collection assemblies are arranged at the outlet ends of the optical channels; each fluorescence collection assembly comprises a focusing lens and a band-pass optical filter which are sequentially arranged in front of a detector; polychromatic light in the flow cell is refracted by the aspheric mirrors and then propagated in the optical channels, passes through the band-pass optical filters, is transmitted in the band-pass range and then is converged to the detector through the focusinglenses, and the polychromatic light outside the band-pass range is reflected back to the optical channels and then is refracted by the aspheric mirrors and then is projected to the flow cell; and theband-pass optical filters matched with the first optical channel and the second optical channel are a first band-pass optical filter and a second band-pass optical filter respectively, and the band-pass ranges are different. The different band-pass ranges can be set, multiple spectrums can be collected at the same time, the structure is simple, use is convenient, the collection efficiency is twotimes that of a single lens, the efficiency is high, and high practicability and wide applicability are achieved.

Description

Technical field [0001] The invention relates to a fluorescence collection structure, in particular to a fluorescence collection and fluorescence separation structure. Background technique [0002] In the medical and biological fields, flow cytometry is usually used to analyze tiny particles such as cells, DNA and bacteria. Flow cytometry and flow cytometry are a kind of quantitative analysis of single cells and other tiny particles at the functional level. Or the sorting detection method can analyze a large number of cells at a high speed (the speed is generally more than 10,000 per second), and can simultaneously detect multiple parameters of a cell. The laser beam is reshaped and irradiated on the sample stream. The sample stream contains the cells to be tested labeled with fluorescent dyes. When the cells to be tested are irradiated by the laser beam, they produce fluorescence and side-scattered light, the resulting fluorescence is very weak. And the fluorescence radiates to ...

AI Technical Summary

Problems solved by technology

[0004] The NA of the microscope objective lens is inversely proportional to the working distance. The working distance above NA 1.0 is basically within 0.5mm. Because the wall thickness of the flow cell on the flow cytometer generally needs to be above 1.4mm (the wall thickness is too small and it is easy to break during installation. and difficult to process), so we can only use the objective lens whose working distance meets the requirements but the NA is generally less than 0.4, so the collected fluorescence signal energy is limited, and the cost of the microscope objective lens is very high

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