Microfluidic cell counting instrument

Abstract

The invention discloses a microfluidic cell counting instrument, which comprises a control system, a touch screen, an automatic sample injection mechanism, a sample pump, a sheath fluid pump, a microfluidic pipeline quick connecting device, a microfluidic cell counting chip and an optical system. According to the microfluidic cell counting instrument, an input instruction is sent to the control system through the touch screen; under the control by the control system, the microfluidic pipeline quick connecting device achieves the connection between a injection needle and the microfluidic cell counting chip; the sample pump and the sheath fluid pump respectively inject a sample and a sheath fluid into the microfluidic cell counting chip; and the optical system detects the microfluidic cell counting chip, converts a received fluorescence signal into an electric signal, sends the electric signal to the control system, performs collection and counting, generates a report and displays the report on the touch screen. According to the invention, the microfluidic cell counting instrument has characteristics of precise counting, simple counting operation, one-key counting within a short timeand high counting efficiency, wherein the chip is disposable so as to avoid the problem of cross contamination; and the microfluidic cell counting instrument can be used for counting the fluorescentcells in a cell sample.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a microfluidic cell counter. Background technique [0002] Today, cell counting is widely used in biology, medicine, teaching, scientific research, food safety and other fields, and is one of the commonly used experimental methods in modern scientific laboratories. For example, biological cell digestion, inoculation and subculture, cell or bacterial density, quantitative calculation of cell reproduction rate, usually requires a large number of cell number calculations, etc.; medical white blood cell count, red blood cell count, circulating tumor cell count, etc.; Milk somatic cell count, bacterial count, etc. will involve the counting of a large number of cells. [0003] The existing cell counting methods mainly include microscopic counting, image cell counting, conductivity counting, and flow cytometry counting, but these counting methods are not accurate enough, have low...

AI Technical Summary

Problems solved by technology

[0003] The existing cell counting methods mainly include microscopic counting, image cell counting, conductivity counting and flow cytometry counting, but these counting methods are not accurate enough, the counting efficiency is low, the operation is complicated, and there is counting cross-contamination

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