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A kind of atropine hapten and its synthesis method, antigen, antibody and application

A synthetic method and atropine technology, which are applied in chemical instruments and methods, material testing products, animal/human proteins, etc., to achieve the effects of low cost, convenient use, and economical speed.

Active Publication Date: 2021-01-12
SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently no reports on atropine hapten and related quick-test products in China

Method used

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  • A kind of atropine hapten and its synthesis method, antigen, antibody and application
  • A kind of atropine hapten and its synthesis method, antigen, antibody and application
  • A kind of atropine hapten and its synthesis method, antigen, antibody and application

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Experimental program
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Effect test

Embodiment 1

[0036] The preparation of embodiment 1 atropine hapten

Embodiment 1-1

[0037] Embodiment 1-1 a kind of synthetic method of atropine hapten, it may further comprise the steps:

[0038]

[0039] (1): Add 2.5g of nortropine alcohol, 2.8g of anhydrous potassium carbonate and 27mL of DMF (N,N-dimethylformamide) into a 100mL single-necked flask and stir evenly, then add 6-bromo-1 - 3.3 g of hexene, stirred and reacted at 80° C. for 6 h (detected on a TLC plate, color developed by potassium permanganate). After the reaction was completed, cool to room temperature, rotate the oil pump to evaporate under reduced pressure, distill off the solvent DMF, add methanol to dissolve and filter out insoluble matter. After methanol was evaporated to dryness, it was purified by column to obtain a brown viscous oil, that is, intermediate 1; the specific chemical reaction formula involved in this step is shown above.

[0040]

[0041] (2): at N 2 Under the atmosphere, 3.0 g of methyl phenylacetate, 2.2 g of ethyl formate, and 60 mL of dichloromethane were adde...

Embodiment 1-2

[0048] Embodiment 1-2 a kind of synthetic method of atropine hapten, it may further comprise the steps:

[0049] (1): Add 5.0g of nortropine alcohol, 11.2g of anhydrous potassium carbonate and 100mL of DMF (N,N-dimethylformamide) into a 250mL single-necked flask and stir evenly, then add 6-bromo-1 - 8.0 g of hexene, stirred and reacted at 80° C. for 6 h (detected on a TLC plate, color developed by potassium permanganate). After the reaction was completed, cool to room temperature, rotate the oil pump to evaporate under reduced pressure, distill off the solvent DMF, add methanol to dissolve and filter out insoluble matter. After methanol was evaporated to dryness, it was purified by column to obtain a brown viscous oil, namely Intermediate 1.

[0050] (2): at N 2 Under the atmosphere, 6.0 g of methyl phenylacetate, 8.8 g of ethyl formate, and 100 mL of dichloromethane were added into a 250 mL single-necked flask, stirred evenly, and cooled to 0°C. While stirring, 12.0 g of t...

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PUM

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Abstract

The invention discloses an atropine hapten and a synthesis method thereof, an antigen, an antibody and an immunodetection device which is convenient to use, rapid, simple and convenient to operate, high in sensitivity and accuracy, and capable of measuring atropine on site. The atropine hapten has a structure as shown in a formula (I) which is described in the specification. The atropine hapten provided by the invention is a proper atropine hapten which is prepared by using nortropine as a raw material and can be more fully exposed to an immune system of an animal. The atropine hapten is coupled with a carrier protein to prepare the artificial antigen; and the atropine artificial antigen is used for immunizing animals, and the monoclonal antibody with high specificity to atropine is prepared by adopting a cell fusion technology.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to atropine hapten and its synthesis method, antigen, antibody and application. Background technique [0002] Atropine is an anticholinergic drug and an M-receptor blocker, which can be used for sedation and pain relief, but also has certain toxicity and may cause central nervous system poisoning. With the rapid development of the economy, people pay more and more attention to the quality of life, so that health care products have a broad market prospect. Driven by interests, some unscrupulous merchants may add atropine in violation of regulations to relieve gastric ulcers. If it is added in excess, it will affect the health of consumers. [0003] At present, the detection methods of atropine at home and abroad mainly include high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D471/08C07K14/765C07K14/77C07K14/795C07K16/44G01N33/94
CPCC07D471/08C07K14/765C07K14/77C07K14/795C07K16/44G01N33/558G01N33/948
Inventor 王西丽付辉申战宾张丹李细清杨星星邓启福魏雄军黎维杨中杨林林
Owner SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
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