Method for determining optimal preservation temperature of sulfur autotrophic denitrifying bacteria biological membrane
A technology of nitrifying bacteria and sulfur autotrophy, applied in the field of environmental engineering, can solve problems such as the impact of sulfur autotrophic denitrifying bacteria biofilm engineering application, the recovery of sulfur autotrophic denitrifying bacteria biofilm activity, etc., to maintain long-term stability operation, shortened start-up time, and reduced operating costs
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Embodiment 1
[0030] Sulfur autotrophic denitrifying bacteria biofilm preservation culture:
[0031] The storage temperature of sulfur autotrophic denitrifier biofilm was set at -20°C, 4°C and 20°C. About 90 biofilms of sulfur autotrophic denitrifying bacteria in the pilot test reaction device were taken out, and the average three equal parts were respectively placed in 500ml serum bottles equipped with 250ml preservation matrix (serum bottles were filled with N in advance). 2 to remove O from the air 2 ), the storage medium is the effluent from the secondary sedimentation tank of the sewage treatment plant, the COD concentration is 40-50mg / L, and the NH 4 + -N concentration is 0.3-0.5mg / L, NO 3 - -N concentration is 8-12mg / L. Serum bottles (three parallel samples were set at each storage temperature) were placed at -20°C, 4°C and 20°C respectively, and kept in the dark.
[0032] During the commissioning and operation of the pilot system, the pH of the effluent fluctuates between 6.2 ...
Embodiment 2
[0044] The activity recovery condition of the sulfur autotrophic denitrifying bacteria biofilm preserved in embodiment 2
[0045] Sulfur autotrophic denitrifying bacteria biofilms from different serum bottles were inoculated into bioreactors (effective volume 20.0L, effective height 150cm) to restore the activity of sulphoautotrophic denitrifying bacteria biofilms. Biofilms of sulfur autotrophic denitrifiers stored at -20°C, 4°C, and 20°C were placed in R1, R2, and R3, respectively. Coarse sand and stones are laid on the bottom 20cm of the bioreactor to support the upper elemental sulfur particles. The upper part of the coarse sand and stones is 100cm elemental sulfur particles with a porosity of about 40%.
Embodiment 3
[0046] Example 3 Biofilm characteristics of sulfur autotrophic denitrifying bacteria after activity recovery
[0047] After 30 days of activity recovery, the biofilm characteristics of sulfur autotrophic denitrifiers in R1, R2 and R3 are shown in Table 2. After the recovery of sulfur autotrophic denitrifier biofilm activity, they were stored at 4°C and 20°C The nitrate nitrogen removal rate of sulfur autotrophic denitrifying bacteria biofilm was lower than that of sulfur autotrophic denitrifying bacteria biofilm before preservation, only the nitrate nitrogen removal rate of sulfur autotrophic denitrifying bacteria biofilm stored at -20℃ The removal rate was the same as before storage. The total nitrogen removal rates of sulfur autotrophic denitrifying bacteria biofilms at different storage temperatures were lower than those before storage. The rate was close to the total nitrogen removal rate of the sulphoautotrophic denitrifying bacteria biofilm before storage, but the total...
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