A method to determine the optimum storage temperature of aerobic granular sludge
An aerobic granular sludge and cell activity technology, applied in the field of environmental engineering, can solve the problems of complex operation and long time, and achieve the effects of shortened activity recovery time, high feasibility, and shortened start-up time
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Embodiment 1
[0025] Embodiment 1: storage condition of aerobic granular sludge
[0026] The storage temperature of the aerobic granular sludge was set at -20 °C, 4 °C and 20 °C. Take out 900ml of the mud-water mixture in the aerobic granular sludge pilot plant, and place three equal parts on average in 1000ml serum bottles with 500ml of preservation matrix. The composition of the preservation matrix is as follows: NaAc 4200mg / L, NH 4 Cl 1100mg / L, K 2 HPO 4 370mg / L, KH 2 PO 4 140mg / L, MgSO 4 440mg / L, KCl 170mg / L, trace element solution 1ml / L; the composition of trace element solution is as follows: FeCl 3 ·6H 2 O1.5g / L,H 3 BO 3 0.15g / L, CuSO 4 ·5H 2 O 0.03g / L, KI 0.03g / L, MnCl 2 4H 2 O 0.12g / L,Na 2 MoO 4 2H 2 O 0.06g / L, ZnSO 4 ·7H 2 O 0.12g / L, CoCl 2 ·6H 2 O 0.15g / L. Preservation matrix COD300mg / L, NH 4 + -N 60mg / L, PO 4 3- -P 8mg / L. Serum bottles (three parallel samples were set at each storage temperature) were placed at -20°C, 4°C and 20°C respectively, and...
Embodiment 2
[0027] Example 2: Characterization of preserved aerobic granular sludge cell state
[0028] Flow cytometry cell state testing conditions are as follows:
[0029] (1) Take 10ml of the aerobic granular sludge mixture stored in the pilot system and at various temperatures for 3 months, dilute to 100ml with phosphate buffer solution with a pH of 7.2, and vortex for 2 minutes in a vortex instrument to make the sludge Broken into flocs and ensure uniform distribution;
[0030] (2) Filter the crushed sample with a nylon membrane with a pore size of 10 μm, take 1.5 ml and place it in a 1.5 ml conical centrifuge tube;
[0031] (3) The sample is centrifuged at 8000rpm / min for 5min;
[0032] (4) Use a pipette gun to absorb the supernatant of the centrifuged sample, leaving about 0.1ml of the sample, use the pre-cooled phosphate buffer to wash the cells, and repeat the centrifugation and washing twice;
[0033] (5) Use a pipette gun to absorb the supernatant of the centrifuged sample, ...
Embodiment 3
[0038] The activity recovery condition of the aerobic granular sludge preserved in embodiment 3
[0039] The aerobic granular sludge from different serum bottles was inoculated in the Sequencing Batch Reactor (SBR) to restore the activity of the aerobic granular sludge; stored at -20°C, 4°C and 20°C The aerobic granular sludge was placed in R1, R2 and R3 respectively. SBR has an effective volume of 10.0L, a drainage ratio of 50%, a reaction period of 3 hours, a static water intake period of 60 minutes, an aeration reaction period of 112 minutes, a sludge settlement period of 3 minutes, and a rapid drainage period of 5 minutes. The content and ratio of air and nitrogen are controlled by the real-time control system to ensure the anaerobic state of the water inlet section and the aerobic state of the reaction section; the SRT is controlled at 25 days.
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