A method for determining the optimum storage temperature of anammox biofilms for sewage treatment
An anaerobic ammonium oxidation and sewage treatment technology, which is applied in the field of environmental engineering to achieve the effect of shortening the start-up time, maintaining long-term stable operation and high feasibility
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Embodiment 1
[0030] Anammox biofilm preservation culture:
[0031] The storage temperature of the anammox biofilm was set at -20°C, 4°C and 20°C. Take out 150 anammox biofilm suspension fillers in the sludge dehydration filtrate treatment tank of the sewage treatment plant, and place three equal parts on average in 1000ml serum bottles equipped with 750ml preservation matrix respectively (serum bottles are pre-filled with N 2 to remove O from the air 2 ), save the matrix as NH 4 Cl and NaNO 2 Provide NH 4 + -N and NO 2 - -N, the remaining ingredients are as follows: KHCO 3 1500mg / L, K 2 HPO 4 15mg / L, MgSO 4 180mg / L, CaCl 2 20mg / L; storage matrix NH 4 + -N concentration is 50mg / L, NO 2 - -N concentration is 75mg / L. Serum bottles (three parallel samples were set at each storage temperature) were placed at -20°C, 4°C and 20°C respectively, and kept in the dark.
[0032] Preserved Anammox Biofilm Cell State Test:
[0033] Anammox biofilms stored at -20°C, 4°C and 20°C wer...
Embodiment 2
[0043] Example 2 The activity recovery of the preserved anammox biofilm
[0044] A sequencing batch reactor is selected, and the specific operating conditions include: Anammox biofilms taken from different serum bottles are inoculated in bioreactors (effective volume 10.0L) to activate anammox biofilms recover. Anammox biofilms stored at -20°C, 4°C, and 20°C were placed in R1, R2, and R3, respectively. The bioreactor is pre-passed with N 2 In order to reduce the inhibition of oxygen on anammox bacteria, the HRT was set to 4h, and the filling ratio of the anammox biofilm suspended filler was 40%.
Embodiment 3
[0045] Example 3 Anammox biofilm characteristics after activity recovery
[0046] After 30 days of activity recovery, the anammox biofilm characteristics in R1, R2 and R3 are shown in Table 2. After recovery of anammox biofilm activity, they were stored anaerobically at 4°C and -20°C The density and thickness of the ammonia oxidation biofilm were close to those of the anammox biofilm before storage, and only the density and thickness of the anammox biofilm stored at 20°C decreased slightly. The biomass of anammox biofilms decreased at different storage temperatures, but after activity recovery, the biomass of anammox biofilms stored at 4°C and -20°C was close to that of anammox biofilms before storage. The biomass of the oxidized biofilm indicated that the anammox biofilm re-adapted to the environment and the biomass increased steadily. Usually the anammox biofilm specific anammox activity and heme c content are 0.27gN / gMLVSS·d and 2.3umol / gVSS respectively, and the anammox b...
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