Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for determining optimal preservation temperature of aerobic denitrifying bacteria for sewage treatment

A technology of aerobic denitrifying bacteria and buffer solution, which is applied in the field of environmental engineering, can solve problems such as the impact of aerobic denitrifying bacteria engineering application, and achieve the effect of maintaining long-term stable operation, high feasibility, and reducing operating costs

Active Publication Date: 2020-04-14
JIANGNAN UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the preservation and recovery of the activity of aerobic denitrifying bacteria, which affects the engineering application of aerobic denitrifying bacteria

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining optimal preservation temperature of aerobic denitrifying bacteria for sewage treatment
  • Method for determining optimal preservation temperature of aerobic denitrifying bacteria for sewage treatment
  • Method for determining optimal preservation temperature of aerobic denitrifying bacteria for sewage treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Determination of Optimum Preservation Temperature for Aerobic Denitrifying Bacteria

[0028] Preservation of aerobic denitrifying bacteria:

[0029] The storage temperatures of aerobic denitrifying bacteria were set at -20°C, 4°C and 20°C. Take out about 180 aerobic denitrifying bacteria polyurethane suspension fillers from the pilot plant reaction device (processing scale: 100t / d), and place three equal parts on average in 1000ml serum bottles equipped with 600ml preservation matrix. The preservation matrix is ​​a sewage treatment plant Anoxic pool effluent (that is, aerobic pool influent, COD concentration is 100-150mg / L, NH 4 + -N concentration is 27.5-38.5mg / L, NO 3 - -N concentration is 4.5-8.0mg / L. Serum bottles (three parallel samples were set at each storage temperature) were placed at -20°C, 4°C and 20°C respectively, and kept in the dark.

[0030] Flow cytometry to test the microbial cell state of aerobic denitrifying bacteria:

[0031] The ae...

Embodiment 2

[0041] Example 2 Verification of the optimum storage temperature for aerobic denitrifying bacteria

[0042] Activity recovery of preserved aerobic denitrifying bacteria: aerobic denitrifying bacteria polyurethane suspension fillers from different serum bottles were inoculated in a bioreactor (effective volume 10.0L) to activate aerobic denitrifying bacteria recover. Aerobic denitrifiers stored at -20°C, 4°C and 20°C were placed in R1, R2 and R3, respectively. The control reactor DO is 4-5mg / L, HRT is 6h, and the filling ratio of polyurethane suspension filler is 35%.

[0043] Characteristics of aerobic denitrifying bacteria after activity recovery:

[0044] After 30 days of activity recovery, the characteristics of aerobic denitrification bacteria in R1, R2 and R3 are shown in Table 2. After the activity recovery of aerobic denitrification bacteria, the removal rate of nitrate nitrogen and total nitrogen removal in R1 and R2 The nitrate nitrogen removal rate and total nitro...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for determining the optimal preservation temperature of aerobic denitrifying bacteria for sewage treatment, and belongs to the technical field of environmental engineering. According to the method disclosed by the invention, the cell activity states of the aerobic denitrifying bacteria stored at different temperatures are determined by utilizing a flow cytometry, and the preservation temperature when the cell activity state is closest to the cell activity state during pilot run of the aerobic denitrifying bacteria is determined as the optimal preservation temperature, and the cell activity state and the performance effect are verified to be reliable after the test data is subjected to activity recovery. According to the method disclosed by the invention, the step of recovering the activity of the aerobic denitrifying bacteria can be omitted, so that a sewage treatment plant ready to adopt the aerobic denitrification technology to efficiently remove nitrate nitrogen and total nitrogen is effectively helped to realize energy conservation and consumption reduction, and the operation cost is reduced; the removal rate of nitrate nitrogen and the removalrate of total nitrogen can reach 90% or above and 88% or above respectively, and meanwhile, the starting time of the aerobic denitrification bacterium engineering application can be effectively shortened, and long-term stable operation of the aerobic denitrification process is maintained; and the method has very high industrial feasibility.

Description

technical field [0001] The invention relates to a method for determining the optimum storage temperature of aerobic denitrifying bacteria for sewage treatment, and belongs to the technical field of environmental engineering. Background technique [0002] The stable and efficient removal of total nitrogen has always been the focus and difficulty in the operation of sewage treatment plants. With the increasing discharge standards of sewage treatment, it is particularly important to develop, promote and apply appropriate nitrogen removal technologies. Due to the limited land use of sewage treatment plants, adding carbon sources to anoxic ponds has become a method for most sewage treatment plants to improve the efficiency of total nitrogen removal. Adopting this method will not only significantly increase the cost of sewage treatment, but also cause pollution in sewage. Slow degradation of organic matter and waste of carbon sources in sludge. Therefore, sewage denitrification t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N15/14
CPCG01N15/1404C02F3/303C02F3/348C02F2101/16G01N15/1459G01N33/1866G01N2015/1006C02F3/1205C12Q1/04
Inventor 王硕石岩吕慧李激王燕
Owner JIANGNAN UNIV