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Beauveria bassiana strain with high pathogenicity on phauda flammans walker larva and application thereof

A technology of vermilion moth and Beauveria bassiana, which can be used in applications, pesticides, fungi, etc., and can solve problems such as threats to the life safety of residents and ecological environment, low control effect, and long damage period.

Active Publication Date: 2020-04-14
广西南宁天鹰有害生物防治有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the long period of occurrence and damage of the pest, and its explosive nature, chemical control has been widely promoted and applied, but the control effect is very little. At the same time, chemical control also threatens the life safety of residents and the ecological environment. Therefore, it is urgent to find a green control technology.
At present, there is no relevant report on the strain of Beauveria bassiana with high pathogenicity to the larvae of Trichoma vermilion

Method used

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  • Beauveria bassiana strain with high pathogenicity on phauda flammans walker larva and application thereof
  • Beauveria bassiana strain with high pathogenicity on phauda flammans walker larva and application thereof
  • Beauveria bassiana strain with high pathogenicity on phauda flammans walker larva and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Isolation and Purification of Beauveria bassiana Strain PfBb

[0014] The strain was isolated and purified from the larvae of the vermilion trichocarpa larva collected in Du'an County, Hechi City, Guangxi Zhuang Autonomous Region. Operate in an ultra-clean bench, immerse the dead worms in 75% alcohol for 30 seconds, take them out, soak them in 0.1% mercuric chloride solution for 3 minutes, wash them with sterile water for 5 times, and put the worms on the sterilized absorbent paper. Dissect it into small pieces, take a piece of worm tissue and place it in a petri dish for 5 days. After 5 days, the mycelium grows centered on the worm body tissue. Pick the thick mycelium and insert it into the inclined test tube as the strain, and cultivate it for 15 days. The separation and purification is successful if the test tube is covered with white spore layer and there is no miscellaneous bacteria. Preserved in the General Microbiology Center of the China Microbiological Culture...

Embodiment 2

[0016] Identification of Beauveria bassiana strain PfBb

[0017] Morphological identification of Beauveria bassiana strain PfBb: cultured at 25°C for 7 days, the diameter of the colony is 23.85 mm, the color of the colony is white on the front and light yellow on the back, and it is a round flat colony. . Conidiospores are single spores, hyaline spherical, conidiophores tufted, terminal with multiple cylindrical spore-forming cells.

[0018] Molecular identification of Beauveria bassiana strain PfBb: the amino acid sequence of the specific ribosomal DNA-ITS sequence is shown in SEQ ID NO.1, and the base number is 549bp.

[0019] The ITS sequence of the strain was compared with the known sequence in NCBI by BLAST. The closest to the strain was Beauveria bassiana, and the ITS sequence homology was 100%. Combined with morphological identification, the strain was identified as Beauveria bassiana Bacteria.

Embodiment 3

[0021] Determination of Toxicity of Beauveria bassiana Strain PfBb to Larvae of Trichoma vermilion

[0022] 1. Experimental method

[0023] In a sterile operating table, use an inoculation loop to scrape conidia into a 50mL small Erlenmeyer flask, add 20mL of distilled water containing 0.05% Tween-80, shake well and count under a microscope with a hemocytometer to obtain the initial concentration , and then diluted to the required concentration. Prepare the spore solution to 1.0×10 9 , 1.0×10 8 , 1.0×10 7 , 1.0×10 6 , 1.0×10 5 There are 5 concentrations of spores / mL. Each concentration is a treatment, and 0.05% Tween-80 solution is used as a control, and each group is repeated 4 times, with 20 heads in each repetition. The immersion method was used to inoculate, the larvae were immersed in the spore liquid for 3 seconds, then taken out, dried at room temperature, and put into the insect culture dish. Put the worms into the insect culture dish sterilized with 75% alcoho...

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Abstract

The invention discloses separation and an application of a Beauveria bassiana strain with high pathogenicity on phauda flammans walker larva. The Beauveria bassiana strain is named as PfBb. The strainis preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.18839. Beauveria bassiana is separated from muscardine worms of phauda flammans walkers for the first time, and toxicity test results show that the strain has strong pathogenicity to phauda flammans walker larva, has the potential of being developed into microbial insecticides and provides technical support for green prevention and control of phauda flammans walkers.

Description

technical field [0001] The invention belongs to the technical field of pest biological control, and in particular relates to a Beauveria bassiana bacterial strain PfBb and its application in the prevention and control of the vermilion hairy moth. Background technique [0002] Phaudaflammans Walker belongs to Lepidoptera Lepidoptera Phaudaflammans Phaudidae Phauda. It mainly harms Ficus plants, among which Ficus benjamina and Ficus microcarpa suffer the most serious damage. In southern China and some countries in East Asia, Ficus is widely distributed and is an essential tree species for urban greening. Therefore, this insect is found in Guangxi, Guangdong, Fujian, Hainan and Taiwan in China as well as India, Vietnam, Thailand, and Malaysia. Countries such as China and Indonesia often break out hazards. In recent years, news reports of its outbreak and harm in southern China have been frequent. The female adults of the vermilion moth like to lay eggs on the young leaves of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCC12N1/145C12R2001/645
Inventor 郑霞林陈学敏王小云陆温
Owner 广西南宁天鹰有害生物防治有限公司
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