Y-shaped joint and method for double-sample co-construction sequencing library

Abstract

The invention discloses a Y-shaped joint and a method for a double-sample co-construction sequencing library. A first Y-shaped joint comprises a first sequence and a second sequence, wherein the firstsequence comprises a P5 sequence, an i5 sequence, an SP1 sequence and an N1 sequence which are connected in sequence, and the second sequence comprises an L1 sequence and an N1c sequence which are connected in sequence. A second Y-shaped joint comprises a third sequence comprising a P7 sequence, an i7 sequence, an SP2 sequence and an N2 sequence which are connected in sequence; and a fourth sequence comprising an L2 sequence and an N2c sequence which are connected in sequence; L1, L2, N1, N1c, N2 and N2c sequences are different from the illumina joint sequence and the human genome sequence and are not complementary with the illumina joint sequence and the human genome sequence; N1 is complementary with N1c, N2 is complementary with N2c, and N1 is different from N2 and N2c and is not complementary with N2 and N2c. The 3' terminal of L1 and the 5' terminal of L2 have complementary regions. By applying the technical scheme provided by the invention, two independently built samples can beconnected together to form a library, and normal sequencing is carried out.

Description

technical field [0001] The present invention relates to the field of biomedical technology, in particular, to a Y-shaped adapter for co-constructing a sequencing library with two samples and a method for co-constructing a sequencing library with two samples. Background technique [0002] As the most mature project for the clinical transformation of genetic testing, there are various methods of non-invasive prenatal testing: such as NGS (Next Generation Sequencing) method, multiplex PCR method, probe method, digital PCR method and chip method, etc., among which multiplex PCR method , probe method, digital PCR method and chip method have the advantages of low cost, short cycle, high sensitivity, etc., especially the multiplex PCR method, chip method and digital PCR method have greatly improved the detection throughput in recent years, but The throughput of NGS detection still cannot be achieved. At present, there are many research articles on these methods, and there are also...

AI Technical Summary

Problems solved by technology

However, considering the cost of sequencing, the cost of single-end sequencing is generally higher than that of paired-end sequencing, and the sample throughput is not high

Taking NextSeq 500 as an example, the maximum number of samples for a single detection is only 96, which has great limitations in application scenarios with a large demand for sample detection

If non-invasive prenatal testing uses paired-end sequencing (PE sequencing) on ​​a higher-throughput sequencing platform (such as illumina HiSeq X-ten or NovaSeq 6000), the number of testing samples can be increased, and the cost per unit of data volume can also be reduced to a certain extent. Reduced, but at the same time, because the analysis only requires single-end sequencing data, it causes a waste of data at the other end

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