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Magnetic field switching rotating part, magnetic field switching device, optical detection device and heat preservation device

A light detection device and switching device technology, applied in the fields of biochemistry, immunity and molecular detection, can solve the problems of low detection efficiency, increase the complexity of the centrifugal microfluidic system, and the error of detection results, and achieve the effect of reducing complexity

Pending Publication Date: 2020-04-14
SUZHOU GUOKE JUNHAO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The position placement of the fixed magnet and the coil setting of the switchable electromagnet are easy to increase the complexity of the centrifugal microfluidic system
Existing magnetic field switching devices can only support one working area of ​​the microfluidic chip, and the detection efficiency is low
In addition, the light detection device of the existing chemiluminescence detector is often set separately from the reaction module of the microfluidic chip. The process is affected by environmental factors and operations, which often leads to large errors in the detection results.

Method used

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  • Magnetic field switching rotating part, magnetic field switching device, optical detection device and heat preservation device
  • Magnetic field switching rotating part, magnetic field switching device, optical detection device and heat preservation device
  • Magnetic field switching rotating part, magnetic field switching device, optical detection device and heat preservation device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] like Figure 1-2 As shown, a magnetic field switching device described in the present invention includes a rotating module 101 that can provide driving force to drive magnetic particles to move in the microfluidic reaction tank and a microfluidic chip seat module 102 placed in the microfluidic reaction tank. The fluidic chip 104, the rotation module 101 provides control power for the magnetic component lever module 103.

[0051] The rotation module 101 includes: a servo motor fixing seat 11, a disk holder fixing frame 12, a disk holder 13, a magnetic ring 14, a pressure plate 15, a servo motor 16, and a magnetic member 17; the servo motor 16 is composed of The servo motor fixing seat 11 fixes it to the rotating module 101, the disk holder 13 above the microfluidic chip holder module 102 is covered with a magnetic ring 14, and the pressure plate 15 is covered above the magnetic ring 14, The disk holder 13 is installed in the disk holder fixing frame 12 and fixed above t...

Embodiment 2

[0055] Procalcitonin PCT detection

[0056] like Figure 4 As shown, 20 microliters of serum is injected into the sample tank 42, and the disc chip 104 is controlled to rotate at 3,000 RPM (acceleration 5,000 RPM / s). The sample is driven by centrifugal force to make it break through the first micro-flow valve 43 and be transferred. To the magnetic microsphere storage tank 44. The disk chip is rotated clockwise and counterclockwise at 5Hz and 135° to mix the sample and magnetic microspheres to achieve the effect of incubation / reaction.

[0057] Further, the magnetic ring 14 of the magnetic force component is controlled to rotate 135°, so that the reacted magnetic microspheres pass through the second microfluidic valve 45 from the magnetic microsphere storage tank 44 and are transferred to the first reagent storage tank 46 . After the cylinder returned to the original position, the disc chip was rotated clockwise and counterclockwise at 10Hz and 55°, so as to mix the reacted m...

Embodiment 3

[0064] Extraction and purification of DNA circulating in peripheral blood

[0065] like Figure 4 As shown, 100 microliters of serum is injected into the sample tank, and the disk chip is rotated at 2,500 RPM (acceleration 4,000 RPM / s). Microsphere storage tank 44. The disk chip is rotated clockwise and counterclockwise at 10Hz and 75° to mix the sample and magnetic microspheres to achieve the effect of incubation / reaction.

[0066] Further, the magnetic ring 14 of the magnetic element is controlled to rotate 120°, so that the reacted magnetic microspheres pass through the second microfluidic valve 45 from the magnetic microsphere storage tank 44 and are transferred to the first reagent storage tank 46 . After the cylinder 31 returned to the original position, the disc chip was rotated clockwise and counterclockwise at 5 Hz and 120°, so as to mix the reacted magnetic microspheres and 80 microliters of lysate to achieve the first lysis effect.

[0067] Further, the magnetic ...

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Abstract

The invention relates to the technical field of detection, and specifically comprises a magnetic field switching rotating part, a magnetic field switching device, an optical detection device and a heat preservation device. The magnetic field switching rotating part comprises a magnetic force part magnetic ring used for containing a micro-fluidic chip, and the magnetic force part magnetic ring comprises at least one magnetic force part and at least one abutting part. The magnetic field switching rotating part does not need to be additionally provided with an electromagnet coil, and the requirement of a fixed magnet device is met; by utilizing the magnetic field switching device, the magnetic particles can be easily controlled, the complexity of a centrifugal microfluidic system is greatly reduced, and the steps of cleaning, cultivating and mixing can be simultaneously executed by matching with a plurality of working areas on the microfluidic chip; the optical detection device and the magnetic field switching device are arranged in the same module, so that the optical detection error is reduced; the heat preservation device comprising the magnetic field switching device can provide aproper reaction temperature for a sample in the micro-fluidic chip.

Description

technical field [0001] The invention relates to the technical fields of biochemistry, immunity and molecular detection, in particular to a magnetic field switching rotary member, a magnetic field switching device, a light detection device and a heat preservation device. Background technique [0002] Due to the large specific surface area and paramagnetism of magnetic particles, they can quickly move (polymerize, disperse) under the action of a magnetic field to improve reaction efficiency and increase detection sensitivity. They are often used in immune and molecular detection reactions: combining antibodies / antigens with magnetic On the particle surface, the magnetic particles are manipulated to perform steps such as incubation, cleaning, elution, and reaction with the sample. However, the use of magnetic particles in centrifugal microfluidic systems is hindered by the driving force of the system itself (centrifugal force, Coriolis force), and it is difficult to manipulate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543B01L3/00
CPCB01L3/5027G01N33/54326
Inventor 林佳慧杨意枫林秋利张寒
Owner SUZHOU GUOKE JUNHAO BIOTECHNOLOGY CO LTD