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Method for detecting norovirus by using nano-enzyme test strip

A nano-enzyme and test strip technology, applied in the field of norovirus detection, can solve the problems of high cost, not simple and fast, low sensitivity, etc. of ELISA kits, and achieve the effects of high reliability, high sensitivity and high sensitivity

Pending Publication Date: 2020-04-14
CHINESE ACAD OF INSPECTION & QUARANTINE +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0009] Antigen detection mainly includes ELISA method, and a large number of commercial kits have also been developed, which can be applied to the screening of a large number of samples in outbreaks. However, ELISA kits are high in cost and low in sensitivity, and require professional technicians to operate, which is not easy and fast enough.
Colloidal gold detection is the most common rapid detection in the market, but its sensitivity is relatively low

Method used

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  • Method for detecting norovirus by using nano-enzyme test strip
  • Method for detecting norovirus by using nano-enzyme test strip
  • Method for detecting norovirus by using nano-enzyme test strip

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Embodiment Construction

[0056] The nanozyme test strip of the present embodiment detects the method for norovirus, comprises the following steps;

[0057] 1. Synthesis of nanozymes;

[0058] Synthesis of nanozymes by hydrothermal method: 0.3g FeCl 3 ·6H 2 O was dissolved in 20mL of ethylene glycol, stirred to dissolve, and then 1.5g of sodium acetate was added. After the mixture was completely dissolved, it was sealed in a high-pressure reactor, and reacted at 200°C for 14 hours. The obtained product was magnetically separated, and the supernatant was discarded. Washed with ethanol for 3 times, and then dried and stored in an electric thermostat oven at 50-60° C. to obtain the nanozyme.

[0059] Among them: sodium acetate, FeCl 3 ·6H 2 O, ethanol, and ethylene glycol were purchased from Beijing Chemical Reagent Factory; the high-temperature oven was purchased from Tianjin Teste.

[0060] Characterization of nanozymes: The prepared nanozymes were characterized by a transmission electron microscop...

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Abstract

The invention discloses a method for detecting norovirus by using a nano-enzyme test strip. The method comprises the following steps: (1), synthesizing nano-enzyme; (2), preparing a nano enzyme-norovirus antibody probe; (3), assembling a nano-enzyme immunochromatography test strip; and (4), detecting the norovirus by the nano-enzyme immunochromatography test strip. The detection sensitivity of thenano-enzyme immunochromatography test strip can be improved by 10 times by being compared with that of a colloidal gold test strip, and the nano-enzyme immunochromatography test strip is high in sensitivity and reliability to norovirus detection and has specific recognition to norovirus. The method for detecting the norovirus by the nano-enzyme test strip has advantages of high simplicity, greatconvenience, quickness and field application of a colloidal gold technology, has the characteristic of high sensitivity, and has the broad development prospects.

Description

technical field [0001] The invention relates to the technical field of norovirus detection. Specifically, it is a method for detecting norovirus with nanozyme test strips. Background technique [0002] Norovirus (also known as Norwalk Virus, Norwalk Viruses), a member of the Caliciviridae family, is one of the main causes of human viral gastroenteritis, accounting for about 18% of diarrhea cases worldwide. In developed countries, noroviruses are considered the most common cause of outbreaks of abacterial diarrhea in children and adults, causing approximately 19-21 million illnesses, 56,000-71,000 hospitalizations, and 570-800 deaths annually in the United States alone. Although rotavirus is considered a major cause of childhood diarrhea in developing countries, norovirus is rapidly becoming the most common cause of severe gastroenteritis in children in countries where rotavirus vaccines have been included in immunization programmes. A recent study estimated that more than ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/558
CPCG01N33/577G01N33/56983G01N33/558
Inventor 刘键段德民张永江
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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