Composition for attracting hermetia illucens for concentrated oviposition and use method of composition
A black soldier fly and composition technology, applied in the field of insect breeding, can solve the problems of difficult collection, quantification and transportation of egg masses, and achieve the effects of improving the lure effect, easier collection, and concentrated egg laying
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Embodiment 1
[0034] Put 20 female and male black soldier fly adults that have been eclosion for 6 days each into a clean and odorless net cage (300mm*300mm*300mm), and place the net cage in a ventilated and well-lit place.
[0035] Using corrugated paper as the egg-laying substrate, five attractant odorants (methyl acrylate, lactic acid, 3-methylindole, n-heptanoic acid, dimethyl disulfide) were prepared into a 10 μg / μL attractant solution with paraffin oil , and take paraffin oil as a control, take 400 μL attractant solution with a pipette gun to the holes and seams of each corrugated paper section (such as figure 1 Shown), spread evenly in the seams of the front and rear sections of each piece of corrugated paper, such as figure 1 As shown, one piece of corrugated paper for each reagent; put the 6 pieces of corrugated paper coated with the above 6 reagents evenly on the bottom of the cage, so that the distance between the corrugated papers should be kept as far as possible to prevent the...
Embodiment 2
[0038] The method is the same as in Example 1, and the odorant is attracted to methyl acrylate, but the surface of the spawning substrate (corrugated paper) is adjusted to 6 colors of red, yellow, green, blue, purple, and black. The results are shown in image 3 .
[0039] from image 3It can be seen that the number of eggs laid by black soldier flies is the highest on red corrugated paper, accounting for 23.83% of the total, which is significantly higher than that on yellow substrate (7.52%), and the difference between the two is significant (Ppurple>black>blue>green>yellow.
Embodiment 3
[0041] The method is the same as in Example 1, the egg-laying substrate is red corrugated paper, and there are 8 corrugated papers in each cage, and the following components or combinations are used to attract odor substances:
[0042] Group A: The odor-attracting substance is a composition of methyl acrylate and lactic acid, and its mass ratio is 3:2;
[0043] Group B: The odor-attracting substance is a composition of methyl acrylate, lactic acid and trimethylindole, and its mass ratio is 3:2:1;
[0044] Group C: methyl acrylate;
[0045] Group D: lactic acid.
[0046] There were two replicates in each cage, for a total of 4 cages.
[0047] Figure 4 The effects of different compositions of attractive odorants on the oviposition selection of black soldier flies, from Figure 4 It can be seen that the composition of several odor substances with attracting effect is obviously better than the single attractant odor substance on the black soldier fly's oviposition. The order ...
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