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Composition for attracting hermetia illucens for concentrated oviposition and use method of composition

A black soldier fly and composition technology, applied in the field of insect breeding, can solve the problems of difficult collection, quantification and transportation of egg masses, and achieve the effects of improving the lure effect, easier collection, and concentrated egg laying

Active Publication Date: 2020-04-17
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are few reports about attracting black soldier flies to lay eggs. Although a small amount of published patents relate to the attractant of black soldier flies, the patent of CN 104798817 A as the application number has announced natural insecticides for gathering a variety of insects. The attractant is composed of animal blood meal, ammonia water, sodium bicarbonate, brown sugar, yellow rice wine, yeast and auxiliary materials; the patents with application numbers CN 103975902 A, CN 104026080 A and CN 103975901 A disclose the use of breeding leftovers to lure wild animals in the wild. Black soldier flies lay eggs, but these methods mainly use the sense of smell of black soldier flies, and the purpose of these methods is only to lure wild black soldier flies to obtain and reproduce wild populations. In the laboratory, there will be problems such as the above, which cannot well solve the bottleneck problem of attracting black soldier flies to concentrate eggs and collect eggs in production practice, and it is difficult to collect, quantify and transport the egg masses scattered around in time.

Method used

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  • Composition for attracting hermetia illucens for concentrated oviposition and use method of composition
  • Composition for attracting hermetia illucens for concentrated oviposition and use method of composition
  • Composition for attracting hermetia illucens for concentrated oviposition and use method of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Put 20 female and male black soldier fly adults that have been eclosion for 6 days each into a clean and odorless net cage (300mm*300mm*300mm), and place the net cage in a ventilated and well-lit place.

[0035] Using corrugated paper as the egg-laying substrate, five attractant odorants (methyl acrylate, lactic acid, 3-methylindole, n-heptanoic acid, dimethyl disulfide) were prepared into a 10 μg / μL attractant solution with paraffin oil , and take paraffin oil as a control, take 400 μL attractant solution with a pipette gun to the holes and seams of each corrugated paper section (such as figure 1 Shown), spread evenly in the seams of the front and rear sections of each piece of corrugated paper, such as figure 1 As shown, one piece of corrugated paper for each reagent; put the 6 pieces of corrugated paper coated with the above 6 reagents evenly on the bottom of the cage, so that the distance between the corrugated papers should be kept as far as possible to prevent the...

Embodiment 2

[0038] The method is the same as in Example 1, and the odorant is attracted to methyl acrylate, but the surface of the spawning substrate (corrugated paper) is adjusted to 6 colors of red, yellow, green, blue, purple, and black. The results are shown in image 3 .

[0039] from image 3It can be seen that the number of eggs laid by black soldier flies is the highest on red corrugated paper, accounting for 23.83% of the total, which is significantly higher than that on yellow substrate (7.52%), and the difference between the two is significant (Ppurple>black>blue>green>yellow.

Embodiment 3

[0041] The method is the same as in Example 1, the egg-laying substrate is red corrugated paper, and there are 8 corrugated papers in each cage, and the following components or combinations are used to attract odor substances:

[0042] Group A: The odor-attracting substance is a composition of methyl acrylate and lactic acid, and its mass ratio is 3:2;

[0043] Group B: The odor-attracting substance is a composition of methyl acrylate, lactic acid and trimethylindole, and its mass ratio is 3:2:1;

[0044] Group C: methyl acrylate;

[0045] Group D: lactic acid.

[0046] There were two replicates in each cage, for a total of 4 cages.

[0047] Figure 4 The effects of different compositions of attractive odorants on the oviposition selection of black soldier flies, from Figure 4 It can be seen that the composition of several odor substances with attracting effect is obviously better than the single attractant odor substance on the black soldier fly's oviposition. The order ...

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Abstract

The invention discloses a composition for attracting hermetia illucens for concentrated oviposition and an application of the composition, and belongs to the technical field of insect breeding. The composition includes an attracting odor substance arranged on an oviposition substrate, and the attracting odor substance includes one or more selected from the group consisting of methyl acrylate, lactic acid, 3-methylindole, n-heptanoic acid and dimethyl disulfide, the odor substance having the attracting effect and oviposition substrate color having the attracting effect are combined to attract the hermetia illucens to crack channels of corrugated paper for oviposition, insect eggs are mainly concentrated in the corrugated paper, so that the oviposition is more concentrated, and the eggs areeasier to collect. The composition provided by the invention has the multiple combined effects of the odor substance, the oviposition substrate color and the crack channels, and can enhance the oviposition attracting effect on the hermetia illucens, thereby improving the concentrated oviposition and egg collection efficiency of the hermetia illucens.

Description

technical field [0001] The invention belongs to the technical field of insect breeding, and in particular relates to a composition for inducing concentrated oviposition of black soldier flies and an application method thereof. Background technique [0002] With the development of the economy and the improvement of people's living standards, the demand for livestock and poultry products continues to increase, and the production scale continues to expand. At the same time as large-scale and intensive farming, the pollution caused by the excrement discharged is also becoming more and more serious. However, livestock and poultry manure is piled up in farms or septic tanks without being used, which not only pollutes the environment, but also causes serious waste of resources. [0003] The black soldier fly Hermetia illucens (Linnaeus, 1758), also known as the bright-spotted flat-horned soldier fly, belongs to the Diptera Soldier Fly family. The insect is widely distributed in mo...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K50/90A23K20/105A23K20/132A23K20/10
CPCA01K67/033A23K20/10A23K20/105A23K20/132A23K50/90
Inventor 周琼裴蕾王鑫桐周鸿林叶碧宇李幸
Owner HUNAN NORMAL UNIVERSITY
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