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The application of salidroside combined with isoniazid to prolong the survival time of Mycobacterium marinum zebrafish model

A technology of mycobacterium and salidroside, which is applied in the fields of application, fish farming, climate change adaptation, etc., can solve the problem that salidroside combined with isoniazid prolongs the survival time of the zebrafish model of Mycobacterium marinum. Application and other issues to achieve the effect of prolonging the survival time

Active Publication Date: 2021-10-22
TIBET UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The use of zebrafish models to screen drugs for the treatment of tuberculosis is of great significance. So far, the inventors have not found any reports on the application of salidroside combined with isoniazid to prolong the survival time of Mycobacterium marinum zebrafish models

Method used

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  • The application of salidroside combined with isoniazid to prolong the survival time of Mycobacterium marinum zebrafish model
  • The application of salidroside combined with isoniazid to prolong the survival time of Mycobacterium marinum zebrafish model
  • The application of salidroside combined with isoniazid to prolong the survival time of Mycobacterium marinum zebrafish model

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Embodiment 1

[0025] The application of salidroside combined with isoniazid to prolong the survival time of Mycobacterium marinum zebrafish model

[0026] Proceed as follows:

[0027] S1. Prepare AB wild-type zebrafish, fertilize the zebrafish on the second day, pull out the plate to collect the fertilized eggs, place the fertilized eggs in a methylene blue solution with a concentration of 0.295 μmol / L, and incubate at 28.5°C. The blue solution is made by dissolving methylene blue in embryo culture water, and the concentration is 0.5 mg / L; after 8 hours, the fertilized eggs are transferred to the mixed solution of salidroside and phenylthiourea for soaking, and the mixed solution is prepared by It is prepared by dissolving phenylthiourea and salidroside in embryo culture water. In the mixed solution, the final concentration of salidroside is 1.5-1.8mM / ml, and the final concentration of phenylthiourea is 0.295μmol / L;

[0028] S2. Calculated according to the fertilization time, the zebrafish...

Embodiment 2

[0039] method

[0040] 1) Place sexually mature Tg(mfap4 EGFP) zebrafish (transgenic zebrafish with green fluorescent protein-labeled macrophages) one male and one female respectively in a mating tank with a baffle inserted in the middle overnight, and the light and dark are automatically controlled for 14 / 10h, the baffle was pulled out after the second daylight, and the zebrafish mated and spawned immediately. Collect fish roe with mesh sieve and rinse with pure water, fish roe is put into methylene blue solution (concentration is 0.5mg / L, is used for inhibiting fungus), after 8 hours, replace with phenylthiourea solution (concentration 0.295 μmol / L, used to inhibit zebrafish melanin). And pick 80 eggs separately and put into the mixed solution of phenylthiourea solution and salidroside solution (the final concentration of salidroside is 1.5-1.8mM / ml, the final concentration of phenylthiourea is 0.295 μ mol / L ), when the 3dfp was hatched, it was recorded as the drug group,...

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Abstract

The invention relates to the technical field of zebrafish models, in particular to the application of salidroside combined with isoniazid to prolong the survival time of Mycobacterium marinum zebrafish models. Salidroside was used to treat fertilized zebrafish eggs, and isoniazid was used to treat zebrafish injected with Mycobacterium marinum. The application process is: provide AB wild-type zebrafish, after zebrafish mating and fertilization, place the fertilized eggs in methylene blue solution, incubate at 28-29°C, and transfer the fertilized eggs to Hongjing after 7-10 hours Soak in the mixed solution of tianosin solution and phenylthiourea solution; calculate according to the fertilization time, inject the Mycobacterium marinum bacteria solution to the zebrafish 3 days after fertilization, and obtain the Mycobacterium marinum zebrafish model; The M. marinum zebrafish model was cultured in isoniazid solution, and the solution was changed every 24 hours until the M. marinum zebrafish died. This study demonstrated that salidroside combined with isoniazid can prolong the survival time of infected zebrafish.

Description

technical field [0001] The invention relates to the technical field of zebrafish models, in particular to the application of salidroside combined with isoniazid to prolong the survival time of Mycobacterium marinum zebrafish models. Background technique [0002] Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis, and it is still one of the most important infectious diseases threatening human life and health. In recent years, the continuous emergence and rapid spread of multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis, as well as the occurrence of co-infection between Mycobacterium tuberculosis and human immunodeficiency virus, have made the prevention and treatment of tuberculosis more difficult. Traditional anti-tuberculosis drugs have been unable to It is imperative to accelerate the development of new anti-tuberculosis drugs to meet clinical needs. [0003] The pathogenic mechanism of tuberculosis is extremely complex....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10A61K49/00
CPCA01K2207/20A01K2227/40A01K2267/0337A61K49/0008A01K61/10Y02A40/81
Inventor 何树梅晏博张舒林
Owner TIBET UNIVERSITY FOR NATIONALITIES
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