Method for establishing metabolic syndrome rat model
A technology for metabolic syndrome and rat models, which is applied in the field of establishing rat models, can solve the problems of high technical cost, low modeling rate, and difficult operation, and achieve high replication success rate, low cost, and short modeling time Effect
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[0014] The present invention provides a method for establishing a metabolic syndrome rat model in specific implementation, including high-fat, high-sugar and high-salt feed and combined streptozotocin. The high-fat, high-sugar and high-salt feed contains the following important components :Lard 15%, egg yolk 10%, cholesterol 2%, crystalline fructose 15%, salt 5%, corn flour 10%, bran 10.5%, bean cake 10.5%, flour 10.5%, cellulose 6%, fish meal 3%, Bone meal 2.5%;
[0015] The method for establishing a rat model of metabolic syndrome is as follows:
[0016] (1) Prepare 80 SPF-grade Wistar male rats. The rats need to be 8 weeks old and have a body weight of 102g-118g;
[0017] (2) Feed the above-mentioned high-fat, high-sugar and high-salt feed;
[0018] (3) Monitor the body weight and food intake of rats in each group every week, observe the coat color and mental status;
[0019] (4) At the age of 16 weeks, after 12 hours of fasting, a one-time intraperitoneal injection combined with s...
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