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Method for establishing metabolic syndrome rat model

A technology for metabolic syndrome and rat models, which is applied in the field of establishing rat models, can solve the problems of high technical cost, low modeling rate, and difficult operation, and achieve high replication success rate, low cost, and short modeling time Effect

Inactive Publication Date: 2020-05-01
李耀华
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Problems solved by technology

[0003] The technical problems to be solved by the present invention are problems such as high cost, unreliability, difficulty in operation and low molding rate of the prior art

Method used

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Embodiment Construction

[0014] When implemented, the present invention provides a method for establishing a rat model of metabolic syndrome, comprising high-fat, high-sugar, and high-salt feed combined with streptozotocin, and the high-fat, high-sugar, and high-salt feed includes the following important components : lard 15%, egg yolk 10%, cholesterol 2%, crystalline fructose 15%, salt 5%, corn flour 10%, bran 10.5%, bean cake 10.5%, flour 10.5%, cellulose 6%, fish meal 3%, Bone meal 2.5%;

[0015] The method for establishing the metabolic syndrome rat model is as follows:

[0016] (1) Prepare 80 SPF grade Wistar male rats, the rats need to be 8 weeks old and have a body weight of 102g-118g;

[0017] (2) Feeding the above-mentioned high-fat, high-sugar and high-salt feed;

[0018] (3) Monitor the body weight and food intake of rats in each group every week, and observe coat color and mental state;

[0019] (4) When feeding to 16 weeks of age, after fasting for 12 hours, a one-time intraperitoneal ...

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Abstract

The invention provides a method for establishing a metabolic syndrome rat model. The method comprises high-fat high-sugar high-salt feed and combined streptozotocin, wherein the high-fat high-sugar high-salt feed comprises the following important components: 15% of lard oil, 10% of egg yolk, 2% of cholesterol, 15% of crystalline fructose, 5% of table salt, 10% of corn flour, 10.5% of bran, 10.5% of bean cake, 10.5% of flour, 6% of cellulose, 3% of fish meal, and 2.5% of bone meal. The method for establishing the metabolic syndrome rat model is as follows: preparing 80 SPF-level Wistar male rats, wherein the rats need to be 8 weeks old and have a body weight of 102 g-118 g; feeding the high-fat high-sugar high-salt feed; weekly monitoring body mass and food intake amount of the rats in eachgroup, and observing hair color and mental status; and performing fasting for 12 h when feeding to 16 weeks old, and then performing one-time intraperitoneal injection with combined streptozotocin of30 mg / kg. The method has stable and reliable performance and high copy success rate.

Description

technical field [0001] The invention relates to a method for establishing a rat model, in particular to a method for establishing a rat model of metabolic syndrome. Background technique [0002] With the improvement of people's living standards and the arrival of an aging society, the proportion of metabolic syndrome (Metabolic Syndrome MS) in the population is increasing, and it has become a major public health problem that threatens human health. Due to many factors such as ethical principles of human medical research and difficulty in obtaining materials, researchers urgently need to construct a stable MS rat model to further study the pathogenesis of MS and the efficacy of drugs. The existing methods for establishing rat models have problems such as high cost, unreliability, inconvenient operation and low modeling rate. Therefore, an economical, reliable, easy-to-operate and high-yield method for establishing a rat model of metabolic syndrome has become an urgent proble...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02A01K2267/03
Inventor 李耀华
Owner 李耀华
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