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Method for preparing pentosan from wheat bran

A technology of wheat bran and pentosan, which is applied in the field of wheat bran utilization, can solve the problems of strong acid corrosion, unfavorable product safety, high cost of steam explosion method, and harsh conditions, so as to promote effective extraction and utilization, and product The effect of high safety and low cost

Inactive Publication Date: 2020-05-01
中华全国供销合作总社天津再生资源研究所
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Problems solved by technology

[0005] There are many side reactions in the acid method and cooking method, and the corrosiveness of strong acid is not conducive to product safety; the cost of steam explosion method is high and the conditions are harsh

Method used

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Embodiment Construction

[0025] Unless otherwise defined, the technical terms used in the following embodiments have the same meaning as commonly understood by those skilled in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are conventional biochemical reagents; the experimental methods, unless otherwise specified, are conventional methods.

[0026] The present invention will be described in detail below in conjunction with examples.

[0027] A method for preparing pentosan from wheat bran, comprising the steps of:

[0028] Extraction of water-soluble pentosans:

[0029] Wheat bran is pulverized, passed through a 35-mesh sieve, heated and refluxed at 80°C with an ethanol solution of 80% by volume to heat-treat the raw material twice, suction filtered, and vacuum-dried. Weigh 10 g of wheat bran after pretreatment, and place 10 g of wheat bran in Extract in hot water at 100°C for 2 times, solid-to-liquid ratio 1:20, extract...

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PUM

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Abstract

The invention provides a method for preparing pentosan from wheat bran. The method comprises the following steps: pulverizing the wheat bran, carrying out heating reflux with an ethanol solution, carrying out suction filtration and drying, carrying out extracting with boiling water, and carrying out centrifuging so as to obtain a supernatant S1 and a precipitate C1; adjusting the pH value of the supernatant S1, adding alpha-amylase, carrying out stirring at a constant temperature, carrying out detecting with iodine water until no color is developed, carrying out cooling, continuing adjusting the pH value of the solution, adding alkaline protease, carrying out stirring and centrifuging so as to obtain a supernatant, adding an ethanol solution into the supernatant, repeatedly carrying out extracting, carrying out centrifuging so as to obtain a precipitate, and carrying out freeze drying treatment so as to obtain water-soluble pentosan; and with NAOH as an extracting agent, performing same steps so as to prepare alkali-soluble pentosan. According to the invention, a water-soluble pentosan extract and an alkali-soluble pentosan extract are prepared by taking the wheat bran as a raw material, respectively taking water and a NaOH solution as extraction solvents and adopting a solvent precipitation method, so the method is simple and convenient to operate, ideal in yield, high in product safety and low in cost.

Description

technical field [0001] The invention belongs to the technical field of wheat bran utilization, in particular to a method for preparing pentosan from wheat bran. Background technique [0002] A large amount of wheat bran is produced in my country every year, and has not yet been effectively utilized as a resource. Most of them are processed in shallow and extensive forms. The utilization efficiency and product added value are low, and a considerable part of the resources is wasted and pollutes the environment. Therefore, the extraction and utilization of active ingredients in wheat bran, and their industrial application have attracted widespread attention. Pentosan is a heterogeneous polysaccharide, which widely exists in wheat bran, and its content is considerable. In the process of wheat processing, the output of bran can account for about one-fifth of the total processing, and studies have found that the content of pentosan in wheat bran can reach nearly 20%. [0003] Pe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/0057
Inventor 赵斌李曼黄继承杜涛宋莉
Owner 中华全国供销合作总社天津再生资源研究所
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