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A kind of Trichoderma, microbial inoculant and application thereof

A microbial agent, the technology of Trichoderma, applied in the field of microorganisms, can solve the problems of peanut nitrate exceeding the standard, low yield, threats to human health, etc., and achieve the effect of improving the growth status and promoting growth

Active Publication Date: 2021-09-10
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the process of peanut planting, there are problems such as low yield, poor growth quality, irregular growth, and rapid deterioration. The long-term use of traditional chemical fertilizers will not only cause soil compaction, but also cause excessive nitrate in peanuts, which threatens the human body. healthy

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  • A kind of Trichoderma, microbial inoculant and application thereof

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preparation example Construction

[0024] According to the present disclosure, the preparation method of the microbial agent may include: inoculating Trichoderma aculeatus with a preservation number of CGMCCNo.19030 into potato dextrose agar solid medium (PDA), culturing in an incubator at 25°C for 7 days, and taking Add 3-4 clumps to the seed bottle. 25°C, 180rpm shaker shake culture for 48h, make seed liquid for use. The seed liquid is added to the seed fermenter in a volume ratio of 1:150 for cultivation, and then added to the fermenter of the chlamydospore fermentation medium according to a volume ratio of 1:20 (by weight, containing 2.5% starch, 1.5% yeast powder, 5.0% corn steep liquor, 0.4% CaCO 3 , 0.01% ZnSO 4 , 0.03% MgSO 4 ), cultured at 28°C for 132 hours, until 90% of the mycelia formed chlamydospores to obtain a chlamydospore fermentation liquid; then the fermentation liquid was adsorbed with diatomaceous earth, centrifuged or plate-and-frame filtered, and dried to form a wettable powder.

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Embodiment 1

[0033] Inoculate the Trichoderma aculeatus with the preservation number CGMCC No.19030 from the preservation slant into the potato dextrose agar solid medium (PDA), cultivate it in a 25°C incubator for 7 days, and take 3-4 bacterial blocks from it and add it to the seed bottle . Shake culture at 25°C and 180rpm for 48h on a shaker to prepare seed solution. Add the seed liquid to the seed fermenter at a volume ratio of 1:150 for cultivation, then add it to the fermenter of the chlamydospore fermentation medium at a volume ratio of 1:20, and cultivate at 28°C for 132 hours until 90% of the hyphae form thick chlamydospores to obtain a chlamydospore fermentation liquid; then use diatomaceous earth to absorb the fermentation liquid, centrifuge or plate and frame filter, and dry to form a wettable powder. The number of viable bacteria of Trichoderma aculeatus in the obtained wettable powder was 5×10 9 CFU / g, this wettable powder is the microbial inoculant of the present embodiment...

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Abstract

The present disclosure relates to a kind of Trichoderma, the classification name of the Trichoderma asperellum (Trichoderma asperellum), and the preservation number of the Trichoderma asperellum is CGMCC No.19030, the Trichoderma asperellum is a very excellent promoting plant growing strains. The present disclosure also relates to a microbial inoculum and the application of the microbial inoculant in promoting plant growth.

Description

technical field [0001] The present application relates to the technical field of microorganisms, in particular, to a Trichoderma, microbial agent and applications thereof. Background technique [0002] Peanut is an agricultural product with high nutritional value, which is deeply loved by the masses. Peanuts are rich in protein, fat and carbohydrates, in addition to minerals and vitamins, suitable for all ages. In addition to its edible value, peanuts can also be used as oil crops, making peanuts into high-grade vegetable oil, making peanuts into peanut cakes for feed, etc. [0003] There is a large gap in my country's existing vegetable oil crops. Peanut, as an important oil crop and economic crop in China, plays an important role in promoting farmers' income and social and economic development. However, in the process of peanut planting, there are problems such as low yield, poor growth quality, irregular growth, and rapid deterioration. The long-term use of traditional ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/38A01P21/00C12R1/885
CPCC12N1/14C12N1/145C12R2001/885
Inventor 吴蓓蕾蒋细良李梅
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI