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Method for rapidly establishing inbred line of hexagrammos otakii

A technology of Otaki hexalis and inbred lines, which is applied in the field of chromosome manipulation, can solve the problems of reducing the survival rate and induction effect, affecting the induction effect, pollution and death of surrounding good eggs, etc., and achieve the effect of improving the fertilization rate and survival rate

Active Publication Date: 2020-05-05
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of gynogenesis induction, the treatment conditions of multi-layer egg masses or egg sheets are not uniform, which affects the induction effect
The induction effect of gynogenesis will also cause some fish eggs to die. The dead eggs inside the egg slices are difficult to clean up, which can easily lead to contamination and death of the surrounding good eggs, which reduces the survival rate and induction effect

Method used

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  • Method for rapidly establishing inbred line of hexagrammos otakii
  • Method for rapidly establishing inbred line of hexagrammos otakii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Sperm and egg collection

[0029] Fish out the male fish, rinse with clean water, dry the water near the genital opening, squeeze the abdomen towards the genital opening, suck the semen into a centrifuge tube, and store it on ice temporarily; select the fish with a swollen and soft abdomen, red and swollen genital pores, and gonad development. For a good female fish, rinse with clean water, dry the water near the genital opening, squeeze the abdomen towards the genital opening, and squeeze the eggs into a clean and smooth container.

[0030] 2) Inactivation of Otaki Hexaline semen

[0031] The semen was diluted 100 times with pre-cooled Ringer's solution, and the dosage was 50,000erg / mm 2 Ultraviolet rays inactivate the sperm, and the inactivated semen is temporarily stored on ice in the dark.

[0032] 3) Artificial insemination

[0033] Add an equal volume of Ringer's solution to the collected fish eggs, wash off the mucus in the eggs, and filter out the eggs tha...

Embodiment 2

[0040] 1) Sperm and egg collection

[0041] Fish out the male fish, rinse with clean water, dry the water near the genital opening, squeeze the abdomen towards the genital opening, suck the semen into a centrifuge tube, and store it on ice temporarily; select the fish with a swollen and soft abdomen, red and swollen genital pores, and gonad development. For a good female fish, rinse with clean water, dry the water near the genital opening, squeeze the abdomen towards the genital opening, and squeeze the eggs into a clean and smooth container.

[0042] 2) Inactivation of Otaki hexaline semen

[0043] The semen was diluted 200 times with pre-cooled Ringer's solution, and the dose was 45,000erg / mm 2 Ultraviolet rays inactivate the sperm, and the inactivated semen is temporarily stored on ice in the dark.

[0044] 3) Artificial insemination

[0045] Add an equal volume of Ringer's solution to the collected fish eggs, wash off the mucus in the eggs, and filter out the eggs that ...

Embodiment 3

[0051] 1) Sperm and egg collection

[0052] Fish out the male fish, rinse with clean water, dry the water near the genital opening, squeeze the abdomen towards the genital opening, suck the semen into a centrifuge tube, and store it on ice temporarily; select the fish with a swollen and soft abdomen, red and swollen genital pores, and gonad development. For a good female fish, rinse with clean water, dry the water near the genital opening, squeeze the abdomen towards the genital opening, and squeeze the eggs into a clean and smooth container.

[0053] 2) Inactivation of Otaki hexaline semen

[0054] The semen was diluted 100 times with pre-cooled Ringer's solution, and the dose was 48,000erg / mm 2 Ultraviolet rays inactivate the sperm, and the inactivated semen is temporarily stored on ice in the dark.

[0055] 3) Artificial insemination

[0056] Add an equal volume of Ringer's solution to the collected fish eggs, wash off the mucus in the eggs, and filter out the eggs that ...

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Abstract

The invention relates to a chromosome operation technology, in particular to a method for efficiently and rapidly establishing an inbred line of hexagrammos otakii. The method comprises the followingsteps: collecting and inactivating seminal fluid of the hexagrammos otakii, performing artificial insemination by utilizing the genetically inactivated sperms and eggs separated one by one, inhibitingsecond polar body emission through hydrostatic pressure, and then inducing gynogenesis to obtain the inbred line of the hexagrammos otakii. By adopting the method, 100% of gynogenesis fries of the hexagrammos otakii can be efficiently, stably and reliably induced and obtained, and the inbred line is rapidly established.

Description

technical field [0001] The invention relates to chromosome manipulation technology, in particular to a method for efficiently and quickly establishing an inbred line of Otaki hexaline fish. Background technique [0002] Hexagrammos otakii, belonging to the order Scorpaeniformes and Hexagrammidae, is a cold-temperate fish, and is an important seawater aquaculture fish in northern my country, Japan and South Korea. Genetic breeding is an effective way to avoid the risk of germplasm degradation brought about by the current multigenerational breeding model, which needs to be carried out urgently. Conventional selective breeding has a long cycle, while gynogenesis technology can quickly obtain inbred lines, which greatly shortens the breeding cycle. Therefore, the development of gynogenesis technology for Otaki Hexaline has very important industrial value. [0003] The technique of gynogenesis has been developed and utilized in many kinds of fishes, but hexaline Otaki has specia...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2227/40
Inventor 吴志昊尤锋宋宗诚王丽娟谭训刚王凌
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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