Application of aloe extract in preparation of medicine for preventing and treating porcine epidemic diarrhea

A porcine epidemic diarrhea and aloe vera extract technology, applied in the field of biotechnology applications, can solve the problems of cumbersome preparation steps, complex ingredients, single ingredients, etc., and achieve the effect of single and simple ingredients, wide range of raw materials, and no toxic and side effects

Active Publication Date: 2020-05-08
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In view of this, the present invention aims at the problems of complicated ingredients and cumbersome preparation steps of traditional Chinese medicine compound, a

Method used

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  • Application of aloe extract in preparation of medicine for preventing and treating porcine epidemic diarrhea
  • Application of aloe extract in preparation of medicine for preventing and treating porcine epidemic diarrhea
  • Application of aloe extract in preparation of medicine for preventing and treating porcine epidemic diarrhea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Aloe extract to Vero cytotoxicity test and the test of inhibiting the proliferation of PEDV in Vero cells

[0035] 1.1 Aloe Vera Extract (AE) To Vero Cytotoxicity Detection Test

[0036] Inoculate 6 × 10 in a 96-well plate 5 Cells / mL cell suspension (100 μL / well), leave a column without inoculating cells. At 37°C, 5% CO 2 cultured until the cells were fully confluent. The culture medium was sucked off, washed 3 times with PBS, the aloe extract was diluted with DMEM to different concentrations, added to a 96-well plate (100 μL / well), and each dilution concentration was replicated 8 times. At 37°C, 5% CO 2 After culturing for 24h and 48h under certain conditions, the drug solution was sucked off, washed 3 times with PBS, and 10% CCK-8 solution was prepared in DMEM and added to a 96-well plate (100 μL / well). 2 Incubate for 1 h under the conditions, measure the absorbance (λ=450nm) with a microplate reader, and calculate the cell viability after 24 h and 48 h...

Embodiment 2

[0069] Example 2 Aloe extract to IPEC-J2 cytotoxicity test and the test of inhibiting the proliferation of PEDV in IPEC-J2 cells

[0070] 2.1 Aloe extract (AE) to IPEC-J2 cytotoxicity detection test

[0071] Inoculate 6 × 10 in a 96-well plate 5 Cells / mL cell suspension (100 μL / well), leave a column without inoculating cells. At 37°C, 5% CO 2 cultured until the cells were fully confluent. The culture medium was aspirated, washed 3 times with PBS, and the drug to be tested (aloe extract (AE)) was diluted with DMEM to different concentrations, added to a 96-well plate (100 μL / well), and each dilution concentration was replicated 8 times. After culturing at 37°C and 5% CO2 for 24h and 48h, the drug solution was sucked off, washed 3 times with PBS, and 10% CCK-8 solution was prepared in DMEM and added to a 96-well plate (100 μL / well). CO 2 Incubate for 1 h under the conditions, measure the absorbance (λ=450nm) with a microplate reader, and calculate the cell viability after 2...

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Abstract

The invention relates to an application of an aloe extract in preparation of a medicine for preventing and treating porcine epidemic diarrhea. An aloe extract preparation and PEDV are mixed and then inoculated into Vero and IPEC-J2 cells, and after cytopathy occurs, an indirect immunofluorescence assay (IFA), western blotting (WB) and a virus titration method are used for detection. Results show that: the aloe extract can effectively inhibit the proliferation of the PEDV in the Vero cells and the IPEC-J2 cells, has a dose-dependent effect, and can completely inhibit the proliferation of the PEDV under a high-dose condition (16mg/ml).

Description

technical field [0001] The invention relates to the application field of biotechnology, in particular to the application of natural plant extracts in the preparation of medicines for preventing and treating porcine epidemic diarrhea. Background technique [0002] Porcine epidemic diarrhea (Porcine epidemic diarrhea, PED) is an acute, highly contagious disease of pigs, and its causative agent, porcine epidemic diarrhea virus (Porcine epidemic diarrhea virus, PEDV), is a single-stranded Alphacoronavirus (KOCHERHANS R, BRIDGEN A, ACKERMANN M, et al. 2001. Completion of the porcine epidemic diabetes coronavirus (PEDV) genome sequence [J]. Virus Genes, 23(2): 137-144.) . PEDV can cause diarrhea, dehydration, and vomiting in newborn piglets, and has a high mortality rate. [0003] In recent years, PED has been widely prevalent in the world, and outbreaks have occurred in China, the United States, South Korea, Canada and other countries, seriously endangering the development of t...

Claims

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Application Information

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IPC IPC(8): A61K36/896A61K36/886A61P1/12A61P31/14
CPCA61K36/896A61K36/886A61P1/12A61P31/14
Inventor 曹永长胡文锋刘元徐志超靳改改吴雨胡学生
Owner SUN YAT SEN UNIV
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