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Application of a CD47 antibody in a liver cancer radiotherapy sensitivity detection kit

A detection kit, 1. CD47 technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of different curative effects of radiotherapy for patients with liver cancer, and achieve the effect of convenient, fast, easy to accept, and stable expression of the detection method

Pending Publication Date: 2020-05-08
上海东方肝胆外科医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the curative effect of radiotherapy in patients with liver cancer is obviously different, so there is an urgent need for a technology that can accurately provide guidance for the treatment of patients with clinical type III and IV tumor thrombi

Method used

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  • Application of a CD47 antibody in a liver cancer radiotherapy sensitivity detection kit
  • Application of a CD47 antibody in a liver cancer radiotherapy sensitivity detection kit
  • Application of a CD47 antibody in a liver cancer radiotherapy sensitivity detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] This embodiment provides an application of a CD47 antibody in a kit for judging the effect of radiotherapy and / or judging whether a tumor patient is suitable for radiotherapy. The CD47 antibody in the present invention can be a monoclonal antibody, a polyclonal antibody, or their effective fragments, and can also be an antibody obtained by various other genetic engineering methods.

[0072] In this embodiment, the immunohistochemical detection kit is taken as an example for illustration.

[0073] In the application of this embodiment, can comprise the making method of paraffin section, comprise steps:

[0074] (i) Sampling, the length of the tissue sample is 3-6 mm, the width is 3-6 mm, and the thickness is 1-3 mm. After sampling, it should be placed in fixative solution within 30 minutes. The amount of fixative solution is about 20 times that of the tissue block, and the fixation time is 24 hours. The fixative solution includes 10% formalin, 4% paraformaldehyde or 10%...

Embodiment 2

[0102] Application of CD47 Antibody in Detection Kit of Radiotherapy Sensitivity of Liver Cancer

[0103] This embodiment provides a liver cancer radiosensitivity detection kit, which contains anti-CD47 immunoglobulin or immunoconjugate, or an active fragment thereof.

[0104] The detection process is as follows:

[0105] Plate preparation:

[0106] 1. Dilute the capture antibody to a working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted capture antibody. Seal the plate and incubate overnight at room temperature.

[0107] 2. Aspirate each well and wash with Wash Buffer, repeat the process twice, a total of 3 washes. Wash by filling each well with a rinse bottle (400 μL), with a squirt bottle, manifold dispenser, or automatic washer. Thorough liquid removal at each step is critical for good performance.

[0108] 3. Block the plate by adding 300 μL of Reagent Diluent to each well. Incubate for at le...

Embodiment 3

[0123] Verification of the application effect of CD47 in the kit

[0124] The effectiveness of the technical solution of the present invention is verified by taking the tissue section of a tumor patient as an example.

[0125] In this example, all patients with liver cancer enrolled in the group underwent surgery after preoperative radiotherapy. The liver cancer tumor tissue resected from the patient was collected, and the expression level of CD47 was detected, and the detection steps were as described in Example 1.

[0126] Judgment of the results of detecting the expression level of CD47 by immunohistochemical method

[0127] Figure 1 shows the results of detecting the expression level of CD47 by immunohistochemical method. Among them, 1A is negative expression (-), 1B is weak positive expression (+), and 1C is strong positive expression (++). The detection results were divided into low expression group and high expression group, represented by + and ++ respectively.

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Abstract

The invention provides an application of a CD47 antibody in a liver cancer radiotherapy sensitivity detection kit. The application has the advantages that (a) the marker for predicting the treatment effect of liver radiotherapy is provided, and the selection of a treatment scheme of a patient is facilitated; (b) sensitivity of a patient to radiotherapy is analyzed in advance, and invalid treatmentis avoided; and (c) the detection method of the peripheral blood CD47 is more convenient and faster, and is more easily accepted by patients.

Description

technical field [0001] The invention relates to the application of a CD47 antibody in a liver cancer radiotherapy sensitivity detection kit, belonging to the field of biological detection. Background technique [0002] Primary liver cancer (hereinafter referred to as liver cancer) is the sixth most common cancer in the world and the third most deadly. In addition, the incidence of liver cancer complicated with portal vein tumor thrombus is higher. Once combined with portal vein tumor thrombus, the prognosis of patients is often poor. Therefore, portal vein tumor thrombus is an important factor affecting the prognosis of liver cancer. In recent years, with the development of science and technology and the improvement of medical level, especially the improvement of surgical methods and the popularization of multidisciplinary diagnosis and treatment concepts, the treatment of liver cancer with portal vein tumor thrombus has made great progress. In 2004, our team proposed the...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/6872G01N33/57438G01N2333/70503G01N2800/52
Inventor 程树群卫旭彪蒋亚波
Owner 上海东方肝胆外科医院
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