Polyoxometallate and chitosan cross-linked supramolecular film as well as synthesis method and application thereof in antibacterial aspect

A polyoxometalate and chitosan cross-linking technology, which is applied in the fields of application, organic chemical methods, botanical equipment and methods, etc., can solve the problems of structural relationship research, etc., and achieve high-efficiency and long-lasting bactericidal effect and good bactericidal effect , the effect of high antibacterial activity

Active Publication Date: 2020-05-15
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, although many efforts have been made in developing PEGylated chitosan materials, to the best of our knowledge, the incorporation of POMs in PEG-grafted chitosan to generate POM-based PEG / CS hybrid polymers and the structural relationship between the two has not been studied in the literature

Method used

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  • Polyoxometallate and chitosan cross-linked supramolecular film as well as synthesis method and application thereof in antibacterial aspect
  • Polyoxometallate and chitosan cross-linked supramolecular film as well as synthesis method and application thereof in antibacterial aspect
  • Polyoxometallate and chitosan cross-linked supramolecular film as well as synthesis method and application thereof in antibacterial aspect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A supramolecular membrane PEG / CS-POM cross-linked by polyoxometalate and chitosan, the synthetic route is shown in figure 1 , the specific preparation method is as follows:

[0038] 1) Preparation of PEGylated chitosan (PEGylated chitosan, PEG / CS):

[0039] An optimized one-step method was used to synthesize PEGylated chitosan. Specifically, low molecular weight 100-500 kDa chitosan (50 mg) was dissolved in 50 mL acetic acid solution (0.5 M), stirred and dissolved at room temperature to obtain an acetic acid solution of chitosan. The pH of the solution was adjusted to 6.0 with 6 M NaOH. Then a 1 mL solution of mPEG-SVA (2000 Da) with a mass concentration of 50 mg / mL was prepared in DMSO. And it is added in the acetic acid solution of chitosan after the above-mentioned adjusted pH value. Stir the reaction at room temperature for 12-24 h. After the reaction, dialyze with ultrapure water for 1-2 days, and use a dialysis bag with a molecular weight cut-off of 3500 Da ...

Embodiment 2

[0052] Antibacterial effect evaluation method 1 agar plate diffusion method:

[0053] First, the antibacterial activity of the material was qualitatively studied by agar plate diffusion test. Before the experiment, the freeze-dried samples were cut into a certain shape (both length and width were 1.5 cm), and the thickness was 0.2 cm. Subsequently, all materials used in the testing process were sterilized under UV radiation for 30 minutes. Fresh bacterial colonies are distributed on nutrient agar (NA) plates on which the material is placed. The plates were incubated at 37 °C for 24 h. Finally, the zone of inhibition (ZOI) was determined for all samples and optical images were recorded. The experiment was repeated three times consecutively under the same conditions. In this experiment, PEGylated chitosan material was used as the blank control group.

[0054] Figure 8 A shows the optical image of the sample co-cultured with Escherichia coli and Staphylococcus aureus for 2...

Embodiment 3

[0056] Antibacterial effect evaluation method 2 Colony forming unit (CFU) method:

[0057] The long-term and cyclic antibacterial properties of the materials were studied by the colony-forming unit method. The prepared samples were placed into the wells of a 24-well plate and washed three times with sterile NaCl solution. Prepare 100 μL with an initial density of 1 x 10 per mL 5 colony forming units (10 5 CFU / mL) of the bacterial suspension, and evenly dispersed on the surface of the material, and then incubated at 37 °C for 2 h. The wells directly inoculated with bacteria without adding samples were used as the blank control group. Subsequently, prepare a series of 10-fold dilutions of bacteria by adding 1 mL of PBS to each well. And 100 μL of the diluted bacterial solution was inoculated on the NA plate, and the bacterial colonies were counted after growing at 37 °C for 16 h. And record its optical image. The determination of bacterial viability was calculated accordi...

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Abstract

The invention discloses a preparation method of a polyoxometallate and chitosan cross-linked supramolecular film. The preparation method comprises the steps: 1) preparing an acetic acid solution of chitosan, and adjusting the pH value of the acetic acid solution to 4.0-6.0; carrying out a stirring reaction on a dimethyl sulfoxide (DMSO) solution of succinimide valerate (mPEG-SVA) at room temperature for 12-24 h, dialyzing with ultrapure water for 1-2 days after the reaction is finished, freeze-drying the dialyzed mixture to obtain pegylated chitosan, and storing for later use; 2) preparing a pegylated chitosan solution, and adjusting the pH value to 3.0-5.0 to obtain a solution A; making the POM in the claim 1 into a solution, and adjusting the pH value to 2.0-4.0 to obtain a solution B; and uniformly mixing the solution A and the solution B in an isopyknic manner, carrying out a stirring reaction at room temperature for 2-3 h, and freeze-drying to obtain the product. According to theionic cross-linked supramolecular film, POMs and PEGylated chitosan are compounded together, perfect combination of efficient antibacterial efficacy and excellent biocompatibility is achieved, and theobtained film is considered to have wide application prospects in the application fields of biological materials, wastewater treatment, food industry, biomedicine, environmental science and the like.

Description

technical field [0001] The invention belongs to the technical field of functionalized polyacid, and specifically relates to a kind of POM compound with excellent bactericidal effect, and PEGylated chitosan with biodegradability and low toxicity to form a supramolecular film in situ after cross-linking, described in detail Its synthesis method and its antibacterial properties were evaluated. The ionically cross-linked supramolecular film combines POM and PEGylated chitosan to achieve a perfect combination of high antibacterial effect and excellent biocompatibility. The obtained supramolecular film material is expected to be used in biological materials, wastewater treatment , food industry and other application fields play a considerable role. Background technique [0002] In recent years, due to delayed wound healing and failure of implantation of biomaterials, diseases caused by bacterial infections are considered to be a fatal threat to human beings, especially the widesp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D213/73C08J5/18C08L5/08C08K3/32A01N25/10A01N43/40A01N59/26A01P1/00
CPCA01N25/10A01N43/40A01N59/26C07B2200/13C07D213/73C08J5/18C08J2305/08C08K2003/329
Inventor 李明雪韩秋霞方颜邢翠丽赵萌
Owner HENAN UNIVERSITY
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