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Rapid detection test paper, preparation method and application thereof

A technology for detecting test strips and test strips, applied in the field of immunochemical in vitro detection, can solve problems such as abnormal detection and dye instability, and achieve the effect of performance optimization

Active Publication Date: 2020-05-15
RUNBIO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention uses microspheres of three primary colors to realize the color development of different colors in the rapid detection reagent / test paper by mixing, and optimizes the performance of the product at the same time, which solves the problem of abnormal detection caused by the instability of the above-mentioned dyes

Method used

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  • Rapid detection test paper, preparation method and application thereof
  • Rapid detection test paper, preparation method and application thereof
  • Rapid detection test paper, preparation method and application thereof

Examples

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Embodiment 1

[0031] This embodiment provides a test strip for early pregnancy detection, including a bottom plate 1, a chromatographic membrane 2, a bonding pad 3, and a single-sided adhesive tape 11, wherein there are 2 lines on the chromatographic membrane: a detection line 5 and a quality control line 6 .

[0032] This reagent is mainly composed of nitrocellulose membrane (chromatographic membrane 2) with mouse anti-human chorionic gonadotropin monoclonal antibody A (test line 5) and goat anti-mouse IgG polyclonal antibody (quality control line 6) on the solid phase 、Glass fiber pre-absorbed with microsphere-labeled mouse anti-human chorionic gonadotropin monoclonal antibody B (binding pad 3)

[0033] 1.1 Preparation of Conjugate Pad 3

[0034] In this example, we choose microspheres of three primary colors to be marked respectively: red, blue and yellow.

[0035] 1.1.1 Microsphere cleaning

[0036] After shaking the microspheres of the three primary colors, take 1 ml (1% concentrati...

Embodiment 2

[0093] This embodiment provides an ovulation detection test strip, comprising a bottom plate (1), a chromatographic membrane (2) and a binding pad (3), as well as a sampling pad (9) and an absorbent pad (10), wherein the chromatographic membrane There are 2 lines: detection line (5) and quality control line (6). The specific preparation method of the detection test strip is as follows:

[0094] The chromatographic membrane 2 of this reagent has a quality control line 6 and a detection line 5, wherein the adsorbed substance of the detection line is mouse anti-human luteinizing hormone (LH) monoclonal antibody A, and the adsorbed substance of the quality control line is goat anti-mouse IgG polyclonal antibody. Conjugate 4 labeled with microspheres is on the binding pad 3 , and the labeled antibody of the microspheres is mouse anti-human luteinizing hormone (LH) monoclonal antibody B.

[0095] 1.1 Chromatographic membrane 2

[0096] Dilute the antibodies from detection line 5 ...

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Abstract

The invention discloses rapid detection test paper which comprises a chromatography membrane (2) and a combination pad (3), and one end of the chromatography membrane (2) is connected with the combination pad (3). A conjugate (4) is sprayed on the combination pad (3). The conjugate (4) contains a marker marked by using microspheres selected from three primary colors, and a detection line (5) and aquality control line (6) are sequentially arranged on the chromatographic membrane (2) along the liquid chromatography direction. According to the test paper, microspheres of different types and / or proportions are used for generating different color marks, so that the sensitivity and the specificity of a product are improved.

Description

technical field [0001] The invention belongs to the technical field of immunochemical in vitro detection, and in particular relates to a rapid detection test paper marked with three primary colors and a preparation method and application thereof. Background technique [0002] Detection reagents / test papers using microspheres as labels have been widely used in various researches, tests, and diagnoses of biology, chemistry, and medicine. To distinguish different analytes, microspheres of different colors will be used to mark them separately, such as red, blue, black, orange, green, yellow, pink, etc. The most commonly used colors in actual use are: blue, red, Green and black. [0003] The dyes embedded in the microspheres are not stable for a long time, and the colored microspheres will be unstable as the storage time prolongs; and the stability of different color dyes is also different. This bead-to-bead / batch-to-batch stability variability and decrease in dye stability ove...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/76G01N33/577G01N33/558
CPCG01N33/558G01N33/577G01N33/76G01N2333/59
Inventor 高克谨林朝琨
Owner RUNBIO BIOTECH CO LTD