Raw material pretreatment method for extracting sophocarpidine from sophora moorcroftiana

Abstract

The invention relates to a raw material pretreatment method for extracting sophocarpidine from sophora moorcroftiana, and belongs to the technical field of biological extraction. The raw material pretreatment method comprises the following steps: crushing raw materials, sieving with a 40-mesh sieve, soaking the raw materials in ethanol with the concentration of 91% or above, stirring and extracting at 40-45 DEG C for 2-3 hours, carrying out hot filtration, and taking filter residues. According to the method, the sophora moorcroftiana raw material is pretreated firstly, impurities with relatively small polarity in the raw material can be removed, an excellent environment can be provided for subsequent extraction of sophocarpidine, and the purity of sophocarpidine can be improved; loss of sophocarpidine is hardly caused; the methodis safe to use and does not influence the environment. The method is particularly suitable for pretreating the roots and branches ofsophora moorcroftiana.

Description

technical field [0001] The invention relates to the technical field of biological extraction, in particular to a raw material pretreatment method for extracting matrine from Sophora japonica. Background technique [0002] Sophora japonica is soft in texture. When it is young, sheep eat it. The crude protein content of its pods is twice that of stems and leaves. It can be used as protein feed in alpine regions. Alkaloids of Sophora japonica are scarce medical intermediates. Its main active ingredients are matrine and oxymatrine, which have anti-inflammatory, anti-virus, and insecticidal effects. They are widely used in pharmaceutical-grade raw materials, plant-derived biological pesticides, Gynecological lotion. [0003] The method of extracting matrine from Sophora japonica is usually the acid water extraction method, that is, adding a certain volume and concentration of acid water directly to the raw material, extracting it at a certain temperature and extraction time, and...

AI Technical Summary

Problems solved by technology

[0005] 1. There is a lot of lignin in the roots and branches of Sophora japonica, while the epidermis contains a lot of pigments such as chlorophyll. The traditional acid water extraction method will dissolve a large amount of impurities such as lignin and chlorophyll while extracting the target substance matrine , becomes the biggest interference factor in the subsequent purification process, which is not conducive to the later purification;

[0006] 2. The acid water extract must be extracted with chloroform, which is potentially harmful in terms of safety;

[0007] 3. Although the extract can be neutralized with acid and alkali after acid water extraction, a large amount of salt substances will be produced, which will cause adverse effects on the environment

Images