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Attenuated strain of duck reovirus and application of attenuated strain

A technology for reovirus and reovirus disease, which is applied to attenuated duck reovirus strains and their application fields, and can solve problems such as economic losses in duck farming, duck lameness and the like

Active Publication Date: 2020-05-19
QILU ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Breeding ducks can see swelling of the hock joints, with yellow or blood-like exudates inside, and in severe cases, there are cheese-like exudates in the joint cavity, and the affected ducks appear lame to varying degrees, causing serious economic losses to the duck industry

Method used

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  • Attenuated strain of duck reovirus and application of attenuated strain
  • Attenuated strain of duck reovirus and application of attenuated strain
  • Attenuated strain of duck reovirus and application of attenuated strain

Examples

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Effect test

preparation example Construction

[0018] 2. The preparation method of duck reovirus disease live vaccine

[0019] Use duck reovirus attenuated Q strain as the virus seed for vaccine production, use microcarrier suspension culture Vero cells, suspension culture BHK cells and CEF cells, LMH cells, DF-1 cells cultured in roller bottles as materials and methods for antigen preparation , Inoculate the well-growing cells with the attenuated strain of duck reovirus virus, and continuously culture and observe for 36-60 hours. When the cell lesion reaches about 80%, harvest the infected virus suspension, add heat-resistant freeze-drying protective agent, and vacuum freeze Dried to make a live vaccine against duck reovirus disease.

[0020] 3. Research on the characteristics of virus strains

[0021] (1) Proliferation of duck reovirus attenuated strain

[0022] Inoculate the attenuated strain of duck reovirus virus into Vero cells or BHK cells that have grown into a good monolayer at 0.5% to 1% inoculum amount, and pl...

Embodiment 1

[0098] Embodiment 1——Cultivation of Duck Reovirus Attenuated Strain

[0099] The virulent strain of the attenuated strain of duck reovirus for breeding comes from a virulent strain of duck reovirus that the inventor isolated and preserved from the diseased Cherry Valley duck in 2017, which is the attenuated starting strain.

[0100] The Vero cells grown into a good monolayer after being cultured for 24 hours were inoculated with a virulent strain of duck reovirus according to the inoculum amount of 0.5% to 1%, and passaged for adaptive proliferation. After the cytopathic changes were stabilized, the passaged poison was purified, and the purified virus was continued to passage to 100 and 120 passages for safety evaluation. The test was as follows:

[0101] The 100th generation and 120th generation poisons were inoculated with large doses of 1-3 days old susceptible ducks, and each duck was injected with 0.5ml intramuscularly, and a normal saline control group was set up. From ...

Embodiment 2

[0103] Embodiment 2——identification of virus strain characteristics

[0104] (1) Proliferation characteristics

[0105] Inoculate the attenuated duck reovirus strain into Vero cells that have grown into a good monolayer at 0.5% to 1% inoculum, and place at 37°C, 5% CO 2 Cultivate statically in an incubator, observe CPE every day, 36 to 60 hours, and the cytopathy can reach about 80%. After freezing and thawing, the virus suspension is harvested and stored at low temperature. According to this method, 3 batches of virus liquids were prepared, and the virus content was measured, all ≥ 10 8.5 TCID 50 / ml.

[0106] (2) Security

[0107] Get 20 1-day-old susceptible ducks, randomly divide them into 2 groups, 10 in each group, inoculate the attenuated strain of duck reovirus in the first group, inject intramuscularly into the legs, each 0.2ml (containing 10 6.0 TCID 50 ), the second group was not inoculated as a control. After 7 days of observation after inoculation, the spir...

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Abstract

The invention relates to an attenuated strain of a duck reovirus and application of the attenuated strain. The attenuated strain Q of the duck reovirus is obtained by attenuating a virulent strain 17117 of the duck reovirus through continuous passage on a Vero cell; a proliferation level on the Vero cell is high; and after being vaccinated, one-day old ducklings have no obvious clinical symptoms and pathological changes, and thus, the attenuated strain is high in safety. By vaccinating the one-day old ducklings with live vaccines prepared from the attenuated strain Q, effective immune protection can be generated.

Description

technical field [0001] The invention relates to a duck reovirus attenuated strain and application thereof, belonging to the field of veterinary biological products. Background technique [0002] Avian reovirus (ARV) is a member of the family Reoviridae and the genus Orthoreovirus. Its genome is a segmented double-stranded RNA with 10 segments. It is commonly found in chickens, turkeys, and ducks. In hosts such as geese, geese and some birds, the clinical features are viral arthritis, enteropathy, tenosynovitis, short stature syndrome, immunosuppressive diseases and malabsorption syndrome. In the first half of 2005, cherry valley meat ducks raised in Shandong, Fujian, Jiangsu, Hebei, Henan and other places in my country successively developed a disease with spleen necrosis as the main lesion, commonly known as "splenic necrosis". In the second half of 2011, a disease characterized by irregular liver necrosis or mixed hemorrhage spots also occurred in cherry valley meat ducks...

Claims

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Application Information

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IPC IPC(8): C12N7/08
CPCC12N7/00C12N2720/12234A61K2039/5254C12N2720/12251Y02A50/30
Inventor 张青婵郭大伟孟照洁彭建云陈俊霞贾爱琴赵妮徐龙涛王蕾张连秀
Owner QILU ANIMAL HEALTH PROD
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