A kind of method and special kit for detecting cnv marker of cattle vamp7 gene

Abstract

The invention discloses a method and a special kit for detecting the CNV marker of cattle VAMP7 gene. The whole genome DNA of the blood of cattle is used as a template, and the CNV region of the cattle VAMP7 gene and a partial fragment of the reference gene BTF3 are respectively amplified by a real-time fluorescent quantitative PCR method. , according to 2*2 ‑ΔΔCt The quantitative results were divided into insertion type, deletion type and normal type, so as to identify the type of copy number variation of cattle VAMP7 gene. The correlation analysis with the production data of Yunling cattle, Xianan cattle, Pinan cattle and Jiaxian Red cattle showed that the cross section height of normal type individuals was significantly higher than that of insertion type and normal type individuals. By detecting the CNV markers closely related to the growth traits of Yunling cattle at the DNA level, the method can be used to quickly establish the dominant population of genetic resources of local cattle breeds such as Yunling cattle, and is beneficial to speeding up the molecular marker-assisted selection breeding work of cattle. Simple, fast, and easy to promote and apply.

Description

technical field [0001] The invention relates to the field of molecular biology detection of livestock, in particular to the use of QPCR to detect the genotype of the copy number variation site of cattle VAMP7 gene and the application of VAMP7 gene CNV marker in molecular marker assisted selection. Background technique [0002] Since the 1980s, with the rapid development of information technology and molecular biotechnology, the research of animal breeding technology has gradually moved from the macroscopic group level to the microscopic molecular level. Compared with traditional breeding methods, animal molecular breeding is a direct The genes for traits are selected at the molecular level, so the accuracy of both selection and breeding is higher. With the continuous maturity and improvement of animal molecular breeding theory and application technology, molecular breeding of beef cattle has made rapid progress. [0003] Copy number variation (Copy Number Variations, CNVs) ...

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Problems solved by technology

At present, there is no literature report on the effect of VAMP7 gene CNV on the growth traits of Yunling cattle

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