Method for preparing paeonia plant root tip chromosome slice

A technology for chromosome preparation and Paeonia lactiflora is applied in the field of chromosome preparation at the root tip of Paeonia lactiflora, can solve the problems of large influence of preparation results, high requirements for preparation conditions, influence on karyotype analysis results, and the like, and achieves low cost , Expand the range of materials, and increase the effect of the concentration of hydrochloric acid
CN111175102AInactive Publication Date: 2020-05-19SHENYANG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHENYANG AGRI UNIV
Publication Date
2020-05-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a chromosome flaking method of a plant and concretely relates to a method for preparing a paeonia plant root tip chromosome slice. Compared with the prior art, the method is characterized in that the material taking range of a root tip is expanded to plants in a herbaceous peony group and in a Chinese peony group, in the dissociation step, a fixed material is put into a dissociation liquid with the volume ratio of absolute ethyl alcohol to 37% hydrochloric acid being 3: 1 to be dissociated at the room temperature; and a low-permeability step in distilled water is alsoadded. Herbaceous peony and Chinese peony seedlings in a field are taken as raw materials, a special dissociation step is adopted, on one hand, the conditions required by the experiment is reduced, the experiment is more convenient; on the other hand, the concentration of hydrochloric acid is properly increased such that the influence of cell walls and cytoplasm on the flaking result is reduced, and meanwhile, the breakage and decomposition of chromosomes under a high-temperature acidic condition are also prevented; after dissociation, hyposmosis enables the chromosomes to be well dispersed, the counting is convenient, and the background is clean.
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Description

technical field

[0001] The invention relates to a method for preparing chromosomes of plants, in particular to a method for preparing chromosomes of root tips of Paeoniae plants. Background technique

[0002] The genus Paeoniae is a shrub or herb of the Paeoniaceae plant, which is divided into three groups: the peony group, the Paeoniae peony group and the North American Paeoniae group, of which there are only two North American Paeoniae groups distributed in western North America; the Paeoniae group is the largest group in the genus, It is a herbaceous type, distributed in Eurasia, and the peony group is a woody type, endemic to my country. Most of the plants in this genus have bright flowers, among which peony and peony are dubbed "King of Flowers" and "Flower Phase" respectively. Because of its rich colors and charming flower shapes, it is widely used in gardens and gardens.

[0003] Chromosomal karyotype analysis is an important indicator of cell taxonomy, and is valuab...

Claims

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