Composite prostate specific antigen kit and use method thereof
A prostate-specific and kit-based technology, which is applied in measurement devices, instruments, scientific instruments, etc., to improve specificity, reduce missed detection and misdiagnosis, and reduce biopsy tests
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Embodiment 1
[0037]The c-PSA calibrator is fully mixed with the c-PSA antigen and the first buffer solution, and the A-F concentration points are 0, 1.5, 3, 10, 30, 100ng / ml, and the label is stored at 2°C, and the validity period is 15 months;
[0038] Wherein, the first buffer solution is prepared by the operation comprising the following steps: accurately weigh the following components by weight: 0.3 parts of potassium dihydrogen phosphate, 1.5 parts of dipotassium hydrogen phosphate, 0.5 parts of potassium chloride, 0.5 parts of hydrogen sulfate Mycin and 190 parts of glycerin were added to 630 parts by weight of newborn bovine serum respectively, stirred at room temperature for 50 minutes, adjusted to pH 7.45, and then pure water was added until the weight of the solution was 1000 parts by weight;
[0039] The configuration method of the c-PSA quality control substance is the same as that of the c-PSA calibrator, with two concentrations of 3.5 and 30ng / ml;
[0040] The c-PSA pretreatm...
Embodiment 2
[0050] The c-PSA calibrator is fully mixed with the c-PSA antigen and the first buffer solution, and the A-F concentration point is 0, 1.5, 3, 10, 30, 100ng / ml, and the label is stored at 4°C, and the validity period is 15 months;
[0051] Wherein, the first buffer solution is prepared by the operation comprising the following steps: accurately weigh the following components by weight: 0.35 parts of potassium dihydrogen phosphate, 2 parts of dipotassium hydrogen phosphate, 1 part of potassium chloride, 1 part of hydrogen sulfate Mycin and 200 parts of glycerin were added to 640 parts by weight of neonatal bovine serum respectively, stirred at room temperature for 60 minutes, adjusted to pH 7.5, and then pure water was added until the weight of the solution was 1000 parts by weight;
[0052] The configuration method of the c-PSA quality control substance is the same as that of the c-PSA calibrator, with two concentrations of 3.5 and 30ng / ml;
[0053] The c-PSA pretreatment solu...
Embodiment 3
[0063] The c-PSA calibrator is fully mixed with the c-PSA antigen and the first buffer solution, and the A-F concentration point is 0, 1.5, 3, 10, 30, 100ng / ml, and the label is stored at 8°C, and the validity period is 15 months;
[0064] Wherein, the first buffer solution is prepared by the operation comprising the following steps: accurately weigh the following components by weight: 0.4 parts of potassium dihydrogen phosphate, 2.5 parts of dipotassium hydrogen phosphate, 1.5 parts of potassium chloride, 1.5 parts of hydrogen sulfate Mymycin and 210 parts of glycerin were respectively added in 650 parts by weight of neonatal bovine serum, stirred at room temperature for 70 minutes, adjusted to pH 7.55, and then pure water was added until the weight of the solution was 1000 parts by weight;
[0065] The configuration method of the c-PSA quality control substance is the same as that of the c-PSA calibrator, with two concentrations of 3.5 and 30ng / ml;
[0066] The c-PSA pretrea...
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