Double-chamber microbial fuel cell and preparation method thereof
A technology of fuel cells and microorganisms, applied in the direction of biochemical fuel cells, etc., can solve problems such as immature microbial fuel cells
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Embodiment 1
[0034] Such as figure 1 As shown, the present invention provides a dual-chamber microbial fuel cell, comprising two independent anode chambers 11 and cathode chambers 12, the bottom of the anode chamber 11 is provided with an anode 2, and the bottom of the cathode chamber 12 is provided with a cathode 3, so The side walls of the anode chamber 11 and the cathode chamber 12 communicate through the proton exchange membrane 4 . Both the anode chamber 11 and the cathode chamber 12 are filled with medium, the anode chamber 11 is inoculated with Rhodopseudomonas bacteria 7 , and the cathode chamber 12 is inoculated with Methanosarcina bacteria 8 . The anode chamber 11 is transparent; the anode 2 and the cathode 3 are respectively connected to the resistor 5 through wires. The Methanosarcina strain 7 is Methanosarcina barkeri, and the Rhodopseudomonas strain 8 is Rhodopseudomonas palustris.
[0035] Both the anode chamber 11 and the cathode chamber 12 are glass bottles, and the mout...
Embodiment 2
[0041] The preparation of embodiment 2 double-chamber microbial fuel cell
[0042] (1) Select two glass bottles with hollow bottoms and frosted openings on the side walls as the anode and cathode chambers, cut the proton exchange membrane and fix it to the circular frosted openings on the side walls of the two glass bottles with stainless steel clips, and use rubber Seal the mouth of the bottle with a rubber stopper; the dimensions of the ITO conductive glass anode and cathode are both 4.8cm×4.8cm; the effective volume of the glass bottle is 30ml, and the area of the circular frosted opening on the side wall of the glass bottle is 2cm 2 , the area of the proton exchange membrane is 3.5cm 2 .
[0043] (2) Activate the ITO conductive glass with boiling 10% hydrogen peroxide for 5 minutes, and then wash it with ultrapure water.
[0044] (3) Wrap the glass bottle obtained in step (1) and the ITO conductive glass in step (2) with tinfoil paper, and place them in a high-temper...
Embodiment 3
[0059] In order to analyze the interaction between the Rhodopseudomonas palustris bacterium and the Methanosarcina barkeri bacterium, a control group experiment was also carried out, including: the Rhodopseudomonas palustris light group, that is, the cathode chamber was not inoculated with the Methanosarcina barkeri bacterium, and other operations were all the same as in Example 1; the Methanosarcina barkeri dark group , that is, the anode chamber is not inoculated with Rhodopseudomonas palustris, and other operations are the same as in Example 1; the sterile light group, that is, the anode chamber and the cathode chamber are not inoculated with bacteria, and other operations are the same as in Example 1.
[0060] The result of embodiment 2 and 3 is as figure 2 As shown in Table 2, it can be seen that the 3 groups of control groups in Example 3 have no obvious electricity production or methane production, and only the system in Example 1 group can produce electricity ( figure...
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