A kind of method and application of extracting triterpenoid terpenoid a from small rhubarb
The technology of a kind of compound, baloneol, which is applied in the field of extracting the new triterpenoid compound baloneol, can solve the problem of low toxicity and side effects, and achieve the effects of easy preparation method, huge economic and social benefits, and abundant raw materials
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Embodiment 1
[0020] In the specific implementation of the present invention, a method for extracting the new triterpene compound sclerophyllin A from small bark bone, using 20 kg of small bark bone as raw material, heating and refluxing ethanol with 5 times the weight of the raw material and a volume concentration of 95% Extracted 3 times, the extraction temperature was 90°C, and the extraction time was 1 hour each time, and the ethanol was recovered under reduced pressure to obtain the extract-like ethanol extract; the extract-like ethanol extract was suspended in 4.8L of distilled water, followed by petroleum Ether and ethyl acetate were extracted three times, 4.8L each time, for 1.5 hours; the ethyl acetate extraction part was separated by silica gel adsorption column chromatography, and the volume ratio was 100:5, 100:20, 100:30 petroleum ether in sequence - Acetone mixed solvent system for gradient elution, each gradient uses 5.0L eluent, the flow rate is 15mLmin -1 , collect 100: 20 ...
Embodiment 2
[0022] The present invention is a method for extracting the new triterpene compound Bacterene A from small bark bone, using 15 kg of small bark bone as raw material, heating and refluxing 3 times with ethanol that is 3 times the weight of the raw material and having a volume concentration of 90%, and extracting The temperature is 95°C, the extraction time is 1 hour each time, and the ethanol is recovered under reduced pressure to obtain the extract-like ethanol extract; the extract-like ethanol extract is suspended in 3.6L of distilled water, and the extract is mixed with petroleum ether and ethyl acetate in turn. Extract 3 times, 3.6L each time, and the time is 1.5 hours; the ethyl acetate extraction part is separated by silica gel adsorption column chromatography, and the volume ratio is 100︰5, 100︰20, 100︰30 petroleum ether-acetone mixed solvent system Carry out gradient elution, each gradient uses 3.7L eluent, the flow rate is 10mLmin -1 , collect 100:20 petroleum ether-ac...
Embodiment 3
[0024] The present invention is a method for extracting new triterpene compound Bacterene A from small bark bone, using 18 kg of small bark bone as raw material, heating and refluxing 3 times with ethanol that is 4 times the weight of the raw material and having a volume concentration of 85%, and extracting The temperature is 92°C, the extraction time is 1 hour each time, and the ethanol is recovered under reduced pressure to obtain the extract-like ethanol extract; the extract-like ethanol extract is suspended in 4.5L of distilled water, and the extract is mixed with petroleum ether and ethyl acetate in sequence. Extract 3 times, 4.5L each time, and the time is 1.5 hours; the ethyl acetate extraction part is separated by silica gel adsorption column chromatography, and the volume ratio is 100︰5, 100︰20, 100︰30 petroleum ether-acetone mixed solvent system Carry out gradient elution, each gradient uses 4.4L eluent, the flow rate is 12mLmin -1 , collect 100:20 petroleum ether-ac...
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