Primer pair and kit for detecting novel coronavirus by isothermal amplification, and preparation method and application of kit

Abstract

The invention discloses a primer pair and kit for detecting the novel coronavirus by isothermal amplification, and a preparation method and application of the kit. The kit comprises amplification reaction liquid, enzyme freeze-dried powder and a chip; the amplification reaction liquid comprises primers, wherein the primers are shown as SEQ ID NO.1-8, and digoxin or fluorescent groups are marked atthe 5'-ends of nucleotide sequences shown as SEQ ID NO.2 / SEQ ID NO.4 / SEQ ID NO.6 / SEQ ID NO.8, and antibodies corresponding to the primers are contained on a test strip and are separately used as separate detection markers. The kit disclosed by the invention can be used to detect three different sections at one time, so that multiple detection can be avoided; detection can be carried out under a constant-temperature condition, so that instrument detection can be carried out in a laboratory, a suspected object can conduct self detection, and thus detection efficiency is improved; the detectiontime is short; one-time detection can be carried out on one person, and detection can be carried out at any time; and the kit has an internal standard, so that extraction efficiency can be effectivelymonitored.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer pair for isothermal amplification and detection of novel coronavirus, a kit, and a preparation method and application of the kit. Background technique [0002] The new coronavirus is transmitted through respiratory droplets and can also be transmitted through close contact; the population is generally susceptible, the elderly and those with underlying diseases are prone to develop severe infection, and children are occasionally affected. However, due to relatively hidden factors such as the initial symptoms of the new coronavirus are not obvious and the incubation period is as long as 14 days, its patients and carriers are often not detected in time. , Rapid, accurate and effective detection of the new coronavirus is a practical need to prevent the epidemic. [0003] Due to the characteristics of the virus itself, in the prevention and control of the new coronavirus, testin...

AI Technical Summary

Problems solved by technology

The use of methods and technologies such as PCR fluorescent probe method and gene sequencing method to detect new coronaviruses requires not only higher-level laboratories, but also higher-level clinical gene amplification laboratories and personnel protection requirements, which are subject to personnel protection requirements. , site, protection, etc.; the number of samples tested by a single laboratory is severely limited every day, and the screening of large-scale populations cannot be completed quickly; in addition, limited by the time and resource costs of amplification / sequencing instruments One-case-one-test, follow-up inspection at any time, increases the time cost of patients' diagnosis, which is not conducive to the control of the epidemic

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