Silicon bead test nucleic acid extraction kit as well as use method and application thereof

Abstract

The invention provides a silicon bead test nucleic acid extraction kit as well as a preparation method and application thereof. The silicon bead test nucleic acid extraction kit includes a lysis solution A, a lysis solution B, a proteinase K solution, an adsorption solution, an adsorption bead suspension, a rinsing solution and an eluent, wherein the lysis solution A, the lysis solution B and theproteinase K solution are mixed to form a lysate. After the lysis solution A, the lysis solution B and the proteinase K solution are mixed in proportion, the use volume of the lysate can be increased,complete immersion of a tested material is facilitated, so that nucleic acids in cells can be fully released; and at the same time, the problems of low lysis efficiency and decomposition in the presence of light caused by interaction between the lysis solution A and the lysis solution B can be solved, the lysate lyses the cells sufficiently and efficiently, the lysis efficiency is high, the nucleic acids with a higher concentration is obtained, improvement of the quality of the nucleic acids is facilitated, and accuracy of subsequent operation is ensured.

Description

technical field [0001] The present invention relates to nucleic acid detection technology, in particular to a nucleic acid extraction kit by silica bead method and its use method and application. Background technique [0002] With the advancement of DNA testing technology, DNA extraction has become a key link affecting the success of DNA testing. How to extract a sufficient amount of high-quality DNA from trace samples has always been a major problem in the field of forensic DNA testing. At present, the main methods of DNA extraction include magnetic bead method based on the principle of adsorption and dissociation, silica bead method, column method and filter membrane method based on molecular weight difference. [0003] The principle of the silica bead method is similar to that of the magnetic bead method, and the nucleic acid extraction process is roughly the same. It is basically divided into four basic steps: (1) Using a strong protein denaturant such as guanidine thio...

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Problems solved by technology

[0004] However, at present, there are few silica bead method nucleic acid extraction kits in the market, and the quality is uneven. Therefore, the market share of the magnetic bead method is larger than that of the silica bead method in general.

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