Simple and efficient tissue culture propagation method for ficus hirta vahl

A simple and simple technology of five-finger peach, which is applied in the field of simple and efficient tissue culture and propagation of five-finger peach, can solve problems such as unsatisfactory experimental results, and achieve the effect of strong root system and high survival rate

Active Publication Date: 2020-06-02
钦州市林业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The applicant adopts the culture medium and related culture conditions described in the invention to carry out bud proliferation and rooting culture of Prunus chinensis, and the experimental results are extremely unsatisfactory

Method used

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  • Simple and efficient tissue culture propagation method for ficus hirta vahl
  • Simple and efficient tissue culture propagation method for ficus hirta vahl
  • Simple and efficient tissue culture propagation method for ficus hirta vahl

Examples

Experimental program
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Effect test

Embodiment 1

[0022] 1) Disinfection of explants: select the seeds of intact and strong Peach chinensis, peel off the outer skin, rinse with water, and dry in the shade indoors; use 0.1% HgCl on an ultra-clean workbench 2 The solution was sterilized for 10 minutes, then washed 5 times with sterile water, and drained for later use.

[0023] 2) Initial culture: inoculate the sterilized explants on the initial culture medium, culture them in the dark for 10 days, and then culture them under natural light at a temperature of 23°C for 15 days to obtain sterile seedlings; the initial culture medium includes the following components : MS medium, sucrose 25g / L, agar powder 3.8g / L, pH=5.8.

[0024] 3) Bud proliferation and rooting culture: Cut the sterile seedlings into 1cm segments with axillary buds, inoculate them on the two-in-one medium for bud proliferation and rooting, and place them at a culture temperature of 23°C with a light intensity of 1000lx and a light time of 12h / Cultivate 20d unde...

Embodiment 2

[0041] 1) Disinfection of explants: Cut the leaves, petioles and stems from the branches of the vigorously growing and free of diseases and insect pests in the same year, rinse with running water first, add diluted detergent to clean, and finally use a brush to remove the hard parts on the body surface. Remove the brush and put it on the ultra-clean workbench. Soak in 75% alcohol for 60s, rinse with sterile water for 3 times, and then wash with 0.1% HgCl 2 The solution was sterilized for 10 minutes, and washed 6 times with sterile water. Cut the sterilized leaves into small pieces of 0.5cm×1.0cm, and cut the stems and petioles into small pieces of 0.5cm.

[0042] 2) Initial culture: inoculate the sterilized explants on the initial culture medium, culture them in the dark for 12 days, and then culture them under natural light at a temperature of 27°C for 20 days to obtain sterile seedlings; the initial culture medium contains the following groups: Divide: MS medium, sucrose 3...

Embodiment 3

[0047] 1) Disinfection of explants: select the seeds of intact and strong Peach chinensis, peel off the outer skin, rinse with water, and dry in the shade indoors; use 0.1% HgCl on an ultra-clean workbench 2 The solution was sterilized for 10 minutes, then washed 5 times with sterile water, and drained for later use.

[0048] 2) Initial culture: Inoculate the sterilized explants on the initial culture medium, culture them in the dark for 11 days, and then culture them under natural light at a temperature of 25°C for 18 days to obtain sterile seedlings; the initial culture medium contains the following groups: Divide: MS medium, sucrose 28g / L, agar powder 4.2g / L, NAA 1.0mg / L, 6-BA 1.0mg / L, PH=5.8.

[0049] 3) Bud proliferation and rooting culture: Cut the sterile seedlings into 2cm segments with axillary buds, inoculate them on the two-in-one medium for bud proliferation and rooting, and place them at a culture temperature of 25°C, with a light intensity of 1200lx and a light t...

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Abstract

The invention discloses a simple and efficient tissue culture propagation method for ficus hirta vahl. The method comprises the steps of: shearing initially cultured aseptic seedlings into 1cm-2cm segments with axillary buds, inoculating the segments with the axillary buds to a bud proliferation-rooting two-in-one culture medium, and carrying out culturing for 25-40 days, so as to obtain rooted seedlings, wherein the two-in-one culture medium contains the following components: an MS improved culture medium, 0.3mg / L-0.6mg / L of 6-BA, 0.1mg / L-0.3mg / L of NAA, 0.1g / L-0.5g / L of activated carbon, 25g / L-30g / L of sucrose, 3.8g / L-4.8g / L of agar powder, and the pH of the two-in-one culture medium is 5.8. By utilizing the two-in-one culture medium, proliferation and rooting of buds can be synchronously carried out, so that the culture period is greatly shortened, and the culture cost is saved.

Description

technical field [0001] The invention belongs to the technical field of asexual propagation of seedlings, and in particular relates to a simple and efficient tissue culture propagation method for Prunus chinensis. Background technique [0002] Ficus hirta Vahl is a woody plant of the genus Ficus in the family Moraceae, also known as Ficus hirta Vahl, Ficus hirta Vahl, and Tubeiqi. . The five-finger peach is propagated through tissue culture, which has the advantages of retaining the excellent characteristics of the five-finger peach, and is not subject to seasonal restrictions on seedling propagation. For example, the invention patent with publication number CN104106468A discloses a tissue culture rapid propagation method of Prunus chinensis, including the following steps: 1) selection and disinfection of explants: get Peach chinensis leaves as explants for disinfection; 2) differentiation of leaves Obtain clustering bud induction culture: the explants after disinfection ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001Y02P60/40
Inventor 陈丽文时群杨利平吴红英李芳菲李清香陈乃明何贵整梁刚蔡林谭冬晓
Owner 钦州市林业科学研究所
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