A method for preparing shellfish high f-value oligopeptides by subcritical water-assisted enzymatic method

Abstract

The invention discloses a method for preparing shellfish high F-value oligopeptides by a subcritical water-assisted enzymatic method, comprising the following steps: 1) subcritical water treatment: homogenate shellfish processing by-products and mix them with water, use subcritical water Extract with critical water and filter to obtain the extract; 2) Enzymolysis: Use endoprotease to enzymolyze the extract obtained in step 1), and then use exoprotease to enzymolyze to obtain enzymolysis; 3) Clarification: Remove the solid particles in the enzymatic hydrolysis solution obtained in step 2) to obtain a clarified enzymatic hydrolysis solution; 4) Decolorization: use activated carbon to decolorize the clarified enzymatic hydrolysis solution obtained in step 3) to obtain a decolorized enzymatic hydrolysis solution; 5) Refining: The decolorized enzymatic hydrolyzate obtained in step 4) is first subjected to ultrafiltration and then nanofiltration to obtain a high F value oligopeptide filtrate; and the high F value oligopeptide filtrate is dried. The invention effectively improves the F value of the product, greatly shortens the production time, reduces energy consumption, and reduces production costs.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing shellfish high F-value oligopeptides by a subcritical water-assisted enzymatic method. Background technique [0002] Chlamys farreri, bay scallop and other scallops are the main species of shellfish farming in my country. After they are processed into frozen scallops and dried scallops, a large number of processing by-products are produced. These by-products are rich in protein, but this is a processing by-product Most of them are processed into feed or discarded, causing serious environmental pollution and waste of resources. Using these shellfish processing by-products to produce high value-added protein or peptide products is an important way to improve resource utilization and reduce environmental pollution. [0003] High F value oligopeptides are oligopeptide mixtures composed of 2-9 amino acid residues obtained by degrading food proteins with p...

AI Technical Summary

Problems solved by technology

[0004] Enzymatic hydrolysis is the main method to produce oligopeptides with high F value. This method has the problems of long enzymatic hydrolysis time, low extraction rate, high enzyme dosage, and high production cost in practical application.

In addition, the F value of the high F value oligopeptide produced by the existing method is difficult to control, and the F value is difficult to improve through the improvement of the enzymatic hydrolysis process, which affects its application in the fields of medicine and medical nutrition