A kit for RNA immunoprecipitation using protein antibodies

Abstract

The invention discloses a kit for performing RNA immunoprecipitation by using protein antibodies, belonging to the technical field of immunoprecipitation. The kit mainly uses the target protein for immunoprecipitation to isolate the RNA bound to the protein. Compared with the existing kits from Millipore, this kit has the advantages of high specificity, high efficiency, short time-consuming, low cost, and easy operation. It is a commonly used reagent in the laboratory, most of which are non-toxic and harmless; first use formalin solution to fix various protein structures to ensure that the protein-RNA complex structure is destroyed, reduce the RNA yield, and at the same time inactivate various nucleases , reducing its degradation of RNA.

Description

technical field [0001] The invention relates to a kit for performing RNA immunoprecipitation by using protein antibodies, belonging to the technical field of immunoprecipitation. Background technique [0002] There are many RNA-binding proteins in the body. After binding to RNA, these proteins will cut or shear the RNA or transport it to a specific site to transcribe into protein to perform specific functions. Under pathological conditions, specific protein-binding RNA will occur Quality and quantity changes, RNA is isolated for follow-up detection such as real-time quantitative PCR or sequencing, which can provide an in-depth understanding of the pathogenesis of the disease, which is conducive to the development of new therapeutic drugs. [0003] At present, there is no domestic company that develops RNA immunoprecipitation kits. If you follow the literature, you will need to spend a lot of time to optimize the experimental conditions. However, the kits of foreign companies...

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Problems solved by technology

[0003] At present, there is no domestic company that develops RNA immunoprecipitation kits. If you follow the literature, you will need to spend a lot of time to optimize the experimental conditions. However, the kits of foreign companies such as Millipore are expensive and have a long delivery cycle, and the operation is cumbersome. Millipore The delivery cycle of the kit is generally 3-4 weeks, and it takes 2-3 days or even longer to complete the entire experiment, and a large amount of organic solvents are used in the experiment process, which is harmful to the human body and the environment. In addition, There are nearly 20 kinds of reagents in the millipore kit, which is a great test for the operator's skills and patience, and the experimental process requires the use of a -80°C ultra-low temperature refrigerator. Since RNA is easy to degrade, long-term operation will reduce its yield. , too many reagents in the experiment process are prone to errors, resulting in inaccurate experimental results, so it is imminent to develop an economical, easy-to-operate, short-time-consuming and stable kit

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