A method for identifying the authenticity of carrot varieties and its special ssr primer combination

A carrot and primer set technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., to achieve the effects of simple operation, protection of rights and interests, and guarantee of authenticity

Active Publication Date: 2021-04-30
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no method for identifying the authenticity of carrot varieties using SSR markers in the current prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for identifying the authenticity of carrot varieties and its special ssr primer combination
  • A method for identifying the authenticity of carrot varieties and its special ssr primer combination
  • A method for identifying the authenticity of carrot varieties and its special ssr primer combination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Acquisition of SSR Primer Combination for Identifying the Authenticity of Carrot Varieties

[0094] 1. Discovery of 24 SSR loci: These loci were obtained by data mining based on the reference genome Daucuscarota, a closely related species of carrot, and the whole genome resequencing data of 24 germplasm resources.

[0095] 1. Different from other molecular marker identification methods, which randomly and blindly select SSR markers, the present invention uses the carrot reference genome, and uses unique and representative resequencing data of 24 carrot varieties (the resequencing results are not disclosed ), through a specific big data analysis method, the SSR loci of the whole genome are analyzed, and a series of screening conditions such as PIC, MAF, and sequence conservation are screened, and finally a set of SSR loci is obtained. Dimension and other methods were used to select the SSR loci that could fully represent the genome of the crop. Through Target-Seq simpli...

Embodiment 2

[0115] This example is the validity test of the SSR primer combination developed in Example 1.

[0116] The 75 carrot varieties tested in this embodiment are all common fine varieties or some imported varieties from abroad, and the sources are all commercially available. The specific varieties are as follows:

[0117]

[0118]

[0119] 1. Obtaining the genomic DNA of the tested carrot varieties

[0120] Adopt the CTAB method to extract the leaves of 75 test carrot varieties respectively (mixing the true leaves of 30 seeds, that is, the true leaves that each variety gets 30 seeds, refers to: the same variety gets the true leaves of 30 different plants. leaf mixture) to obtain the genomic DNA of the tested carrot variety.

[0121] The operation of the above CTAB method is as follows:

[0122]The leaves of the above-mentioned 75 varieties at the seedling stage were picked respectively, and placed in a freeze dryer (CoolSafe 55-4) for dehydration; Add 800 μL of CTAB extr...

Embodiment 3

[0139] This embodiment is a method for detecting whether the carrot variety to be tested belongs to the 75 carrot varieties for testing. The variety of the carrot to be tested is unknown, and it is necessary to obtain whether the carrot variety to be tested is one of the 75 varieties through the detection method of this embodiment. one of the species.

[0140] 1. Obtaining the genomic DNA of the carrot variety to be tested

[0141] The leaves of the carrot varieties to be tested were taken from the experimental base of the Vegetable Research Center of Beijing Academy of Agriculture and Forestry Sciences.

[0142] According to the method of step 1 in Example 2, replace "the leaves of the carrot variety to be tested" with "the leaves of the carrot variety to be tested", and keep other steps unchanged to obtain the genomic DNA of the carrot variety to be tested.

[0143] 2. Configuration of SSR primers and PCR reaction system

[0144] According to the method of step 2 in Exampl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of molecular markers and detection thereof, in particular to a method for identifying the authenticity of carrot varieties and a combination of special SSR primers. The SSR loci for identifying the authenticity of carrot varieties were obtained by data mining based on the carrot reference genome Daucus carota and the whole genome resequencing data of 36 germplasm resources; the above SSR primer combinations were selected from: the first SSR primer pair to the twentieth Four pairs of SSR primers, respectively used for PCR amplification of the above-mentioned first SSR site to the twenty-fourth SSR site, preferably respectively with the homology of the nucleotide sequence of SEQ ID NO: 1‑48 in the sequence listing 85%‑100% nucleotide sequence. The invention can be used for the authenticity identification of the whole life cycle of carrot varieties starting from seeds, and provides technical support for the protection of carrot germplasm resources and new varieties.

Description

technical field [0001] The invention belongs to the field of molecular markers and detection thereof, in particular to a method for identifying the authenticity of carrot varieties and a combination of special SSR primers. Background technique [0002] Carrot is an Umbelliferae plant native to Central Asia. It has been cultivated for more than 4,000 years. It was widely cultivated in my country by Zhang Qian of the Han Dynasty when he was sent to the Western Regions. At the same time, carrot is also a worldwide vegetable. It is very popular all over the world. [0003] There are few varieties and subspecies of carrots. In the genus Carrot of Umbelliferae, there is only one kind of wild carrot. Cultivated carrots are considered to be varieties of wild carrots. From another perspective, the genetic diversity of carrots is not rich. The differences among varieties of carrots cultivated all over the world may not be large, especially the introduction and domestication work betwe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 温常龙杨静静梁毅张建罗江李晓杰
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap