Earthworm characteristic polypeptides and application thereof in species identification of earthworm medicinal materials

Abstract

The invention discloses a group of earthworm characteristic polypeptides and application thereof in species identification of earthworm medicinal materials. The five characteristic polypeptides have good specificity and stability, can be stably detected in earthworm samples of corresponding species, and cannot be detected in non-corresponding species of earthworms including pheretima guillelmi, pheretima californicum, pheretima aspergillum, pheretima fargesii and other species. The five characteristic polypeptides have excellent representativeness, can distinguish most of earthworm medicinal material species in the market, have the certified product identification rate of 96%, and have good application prospects.

Description

technical field [0001] The invention belongs to the identification technology of Chinese medicinal materials, in particular to a group of earthworm characteristic polypeptides and its application in the identification of earthworm medicinal materials. Background technique [0002] Earthworm is a commonly used animal medicine, which has the effects of clearing heat and relieving convulsions, calming liver and quenching wind, dredging meridian and activating collaterals, relieving asthma and diuresis. The 2015 edition of "Chinese Pharmacopoeia" stipulates that earthworms are Pheretima aspergillum E.Perrier, P.vulgaris Chen, P.guillelmi Michaelsen or Pectinifera Michaelsen. dry body [1] . However, there are many kinds of earthworms. As of 2010, there are 328 kinds of land earthworms in China. [2] . In addition, the shapes of earthworms are relatively similar, and it is difficult to classify and identify them with the naked eye. Pharmacists cannot distinguish the original an...

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Problems solved by technology

[0004] The lack of specificity and specificity of microscopy and thin-layer chromatography (TCL) makes it difficult to identify and distinguish different earthworm species

The method of identification based on the morphological traits of earthworms is not reliable. On the one hand, the morphological characteristics of earthworms will change at different ages and different reproductive stages [3] ; On the other hand, the main distinguishing features such as internal organs, male pores, and mating cavities are often damaged during processing, and body color and surface texture features often disappear or change when drying [4]

Although the DNA molecular identification method is accurate, the DNA will be seriously degraded during the cleaning, drying, storage and other processes of the medicinal materials, as well as the exogenous DNA contamination caused by microorganisms, which will lead to low content and low quality of the extracted DNA. Difficult for subsequent molecular identification [9]

On the other hand, traditional Chinese medicine is finally administered in the form of Chinese patent medicine, decoction pieces, and formula granules. In these applications, the morphology and DNA are damaged, and the above-mentioned conventional methods cannot be well applied.

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