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Fully human antagonistic antibody screened from scfv phage antibody library targeting thy-1/cd90

A THY-1, CD90 technology, applied in the field of antagonistic antibodies, to achieve the effect of low cost and rapid antibody screening

Active Publication Date: 2022-01-04
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Despite the above reports, there has been no literature on the screening of inhibitor antibodies targeting THY-1 from the ScFv phage antibody library using phage display and other technologies

Method used

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  • Fully human antagonistic antibody screened from scfv phage antibody library targeting thy-1/cd90
  • Fully human antagonistic antibody screened from scfv phage antibody library targeting thy-1/cd90
  • Fully human antagonistic antibody screened from scfv phage antibody library targeting thy-1/cd90

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Embodiment Construction

[0025] see figure 1 with figure 2 , a fully human antagonistic antibody screened from the ScFv phage antibody library with THY-1 / CD90 as the target, the fully human antagonistic antibody is a single-chain antibody, and the fully human antagonistic antibody heavy chain DNA The sequence is shown in SEQ ID NO:1, the light chain DNA sequence is shown in SEQ ID NO:2, the heavy chain amino acid sequence is shown in SEQ ID NO:3, and the light chain amino acid sequence is shown in SEQ ID NO:4.

[0026] Preferably: the heavy chain DNA sequence SEQ ID NO:1 is used to encode the heavy chain amino acid sequence SEQ ID NO:3, and the light chain DNA sequence SEQ ID NO:2 is used to encode the light chain amino acid sequence SEQ ID NO:4.

[0027] As preferred: determine the DNA sequence and amino acid sequence of the fully human antagonistic antibody by sequencing, and determine the DNA sequence and amino acid sequence of the fully human antagonistic antibody respectively, so that the obtai...

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Abstract

The invention discloses a fully human antagonistic antibody screened from a ScFv phage antibody library with THY-1 / CD90 as a target, the fully human antagonistic antibody is a single-chain antibody, and the heavy chain DNA sequence of the fully human antagonistic antibody As shown in SEQ ID NO:1, the light chain DNA sequence is shown in SEQ ID NO:2, the heavy chain amino acid sequence is shown in SEQ ID NO:3, and the light chain amino acid sequence is shown in SEQ ID NO:4. According to the above technical scheme, the fully human antagonistic antibody targeting THY-1 / CD90 is screened from the human ScFv phage antibody library, and its DNA sequence and amino acid sequence are respectively determined. The fully human antagonistic antibody obtained by this method has the target Specificity towards THY‑1 / CD90. The antibody produced in this way can overcome the shortcomings of hybridoma technology to produce antibodies, and can also achieve the purpose of high-throughput and rapid screening of antibodies, and the price is low.

Description

technical field [0001] The invention relates to the technical field of antagonistic antibodies, in particular to a fully human antagonistic antibody screened from a ScFv phage antibody library with THY-1 / CD90 as the target. Background technique [0002] THY-1 (thymocyte antigen-1), also known as CD90, is a highly conserved glycoprotein. At present, it has been found on the surface of nerve cells, thymocytes, fibroblast subsets, endothelial cells, glomerular mesangial cells, and hematopoietic stem cells. THY-1 can regulate cell-cell and cell-cytoplasmic reactions, and plays an important role in nerve regeneration and metastasis, inflammation and fibrosis. Lee reported that THY-1 is a novel marker of inflammatory cytokines promoting angiogenesis; Bradley JE and Rege TA two research groups reported that THY-1 is closely related to angiogenesis in some diseases. [0003] In recent years, some literatures have reported that inhibitors or activators targeting the natural ligands...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/28C12N15/13G01N33/569
CPCC07K16/2803G01N33/56983C07K2317/24C07K2317/622
Inventor 叶辉向敏胡煊煊褚晓颖李美琪汪子晗万乐予桑侃如杜邦
Owner WENZHOU MEDICAL UNIV
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