Rapid molecular detection method for pathogenic bacterium colletotrichum siamese of colletotrichum leaf disease of rubber trees, and application of primer

A technology for rubber tree anthracnose and pathogenic bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, and microorganism measurement/inspection, can solve the problems that there are no composite species-specific primers and PCR detection methods, etc., to achieve Rapid and efficient identification of bacterial species, shortened identification time, early diagnosis and timely prevention, reliable results

Pending Publication Date: 2020-06-23
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, only the specific primers and PCR detection methods for the compound species of rubber tree anthracnose, G. anthracnose and Ant

Method used

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  • Rapid molecular detection method for pathogenic bacterium colletotrichum siamese of colletotrichum leaf disease of rubber trees, and application of primer
  • Rapid molecular detection method for pathogenic bacterium colletotrichum siamese of colletotrichum leaf disease of rubber trees, and application of primer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, be used for the acquisition of the Siamese anthracis primer that detects rapidly

[0021] The ApMat gene sequence of different anthrax species obtained by sequencing in our laboratory was compared with the ApMat gene sequence registered in the NCBI database, and specific primers were designed according to the difference between Siamese anthrax and other species, and the obtained primers were the invention. The primers, the primer sequence is upstream primer CSAMPF: 5'-AGTCGTGACAGCGGCTGTGT-3' (sequence 1 in the sequence listing); downstream primer CSAMPR2: 5'-ATTCCCTTCCCGCATGCTGC-3' (sequence 2 in the sequence listing).

[0022] Two, the present invention is used for the effect verification of the rapid detection primer of Siamese anthracnose

[0023] 1. Primer synthesis

[0024] Upstream primer CSAMPF: 5'-AGTCGTGACAGCGGCTGTGT-3' (sequence 1 in the sequence listing); downstream primer CSAMPR2: 5'-ATTCCCTTCCCGCATGCTGC-3' (sequence 2 in the sequence listing)...

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Abstract

The invention discloses a special primer for identifying a pathogenic bacterium colletotrichum siamese of a colletotrichum leaf disease of rubber trees, a rapid molecular detection method of the colletotrichum siamese, and an application of the primer. The special primer for identifying the colletotrichum siamese is a primer pair consisting of a sequence 1 and a sequence 2. According to the rapidmolecular detection method, a PCR reaction is carried out on DNA contained in a sample, and when a PCR product presents a 416bp specific product, the sample is the pathogenic bacterium colletotrichumsiamese. The method has simple operation and high specificity, and the lowest detection content of fungus DNA is 10pg.

Description

technical field [0001] The invention belongs to the technical field of pathogen detection of rubber tree anthracnose, and in particular relates to a method and primers for detecting Siamese anthracnose bacteria carried by rubber tree leaves. Background technique [0002] Natural rubber is one of the four major industrial raw materials in the world. my country is the world's largest consumer of natural rubber. Although it is also one of the producing countries, it is far from meeting domestic demand. How to ensure the stability and improvement of natural rubber production is an urgent problem to be solved at present. [0003] Anthracnose (Colletotrichum leafdisease, CLD) caused by anthracnose fungus (Colletotrichum spp.) is an important disease threatening the natural rubber industry. The disease can occur in every physiological period of the rubber tree, and mainly damages the leaves of the rubber tree, causing repeated defoliation and sparse canopy of the rubber tree, affe...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/04C12N15/11C12R1/645
CPCC12Q1/6895
Inventor 刘先宝李博勋黄贵修郑肖兰蔡吉苗江涛冯艳丽
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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