Micro-fluidic chip applied to capture and screening of single cells, and application thereof

Abstract

The invention provides a micro-fluidic chip applied to capture and screening of single cells, and an application thereof. The micro-fluidic chip comprises four layers of structures which are successively stacked together and mutually sealed and are respectively a micro-valve control layer, a micro-valve film layer, a substrate with a flow way and a micro-pore array and a detection release layer from top to bottom. Through introduction of a micro-processing technology and a micro-fluidic technology, the multi-layer and multi-functional micro-fluidic chip is developed, capture, identification, culture and release of cells are realized, a new method is provided for cell screening, and hyper-secretion cells can be obtained more quickly, accurately and conveniently.

Description

technical field [0001] The invention relates to a microfluidic chip for single cell capture and screening and its application. Background technique [0002] Monoclonal antibody has high specificity and high targeting, and has become an important means of tumor targeted therapy and probe detection. At present, mammalian expression systems for producing monoclonal antibodies include CHO cells, NSO cells, and hybridoma cells. During the long-term culture process, the genes expressing antibody heavy and light chains on chromosomes are deleted or rearranged, resulting in the loss of the ability of cells to secrete specific antibodies. The specific mechanism is still unclear. High-yielding cells that secrete target protein molecules tend to have lower proliferation efficiency. However, unstable heterogeneous hybridoma cells proliferate faster, and their proportion continues to expand, which affects the secretion efficiency of antibodies and becomes the key to increasing antibody ...

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[0010] At present, there is no related technical report that can integrate

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