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Bacillus velezensis strain and application thereof

A technology of Bacillus velesi and Bacillus, applied in the application, bacteria, microorganism and other directions, can solve the problems of ecological environment pollution, resource waste and other problems, achieve the effect of no sewage discharge, reduce pollution, and realize the effect of recycling

Active Publication Date: 2020-06-30
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the tea dregs are discarded, which not only causes waste of resources, but also pollutes the ecological environment.

Method used

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  • Bacillus velezensis strain and application thereof
  • Bacillus velezensis strain and application thereof
  • Bacillus velezensis strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Process flow:

[0031] Sample collection→sample pretreatment→enrichment culture→selection of high-temperature-resistant strains→determination of growth performance→determination of optimal growth pH value→determination of optimal growth temperature→observation of bacterial morphology→obtaining Bacillus velesii ( Bacillus Velezensis ) Fb.

[0032] Medium:

[0033] (1) LB culture medium (L): Yeast extract powder 10g, peptone 10g, beef extract 5g, NaCl 10g, pH6.0, add distilled water to 1L, sterilize at 121°C for 30min.

[0034] (2) Purified solid medium: add 20g / L agar powder to the LB culture medium, and sterilize at 121°C for 30min.

[0035] (3) Separation of solid medium: Take 50g of fresh tea residue and add 500ml of distilled water to cook for 30min, add 20g of agar powder to the filtered tea residue, and sterilize at 121°C for 30min.

[0036] (3) Liquid fermentation medium: yeast extract powder 10g, peptone 10g, beef extract 5g, NaCl 10g, pH 6.0, add distilled...

Embodiment 2

[0049]Obtain a strain of Bacillus Veles by the separation and screening method described in Example 1 ( Bacillus velezensis ) Fb. The identification results are as follows:

[0050] (1) Observe the colony shape and strain shape of Fb. The Fb strain was cultured in LB medium at 45°C for 16 hours. The colony shape is characterized by rod-shaped bacteria, 0.4-0.6μm×0.9-4.0μm, single or paired arrangement, spores Nearly round, near-terminal, with enlarged cysts, belonging to Bacillus Veles, Gram-positive, see details Figure 1-2 .

[0051] It has been tested that the Bacillus Veles can withstand a high temperature of 55°C, and the optimum growth temperature is 45°C, and the optimum growth pH value is 3.0-7.6.

[0052] (2) Physiological and biochemical characteristics of the strain:

[0053]

[0054] (3) Molecular identification of strains

[0055] The sequencing results showed that the 16S rDNA sequence of the strain Fb was 1440 bp long. The results of the Blast program a...

Embodiment 3

[0057] Embodiment 3 Veles bacillus ( Bacillus velezensis ) The preparation method of Fb bacterial powder

[0058] (1) Strain activation:

[0059] Bacillus Velez stored on a slope at 4°C ( Bacillus velezensis ) Fb activation, respectively streaked on the corresponding solid medium, cultured at 45°C for 16h;

[0060] The source of the strain: Bacillus velei ( Bacillus velezensis ) Fb, obtained by self-screening, was deposited in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the number is CGMCC No.19438;

[0061] (2) Seed cultivation:

[0062] Pick the activated colony, Bacillus velei ( Bacillus velezensis ) Fb was inoculated in the seed medium for seed culture, and cultured on a shaker at 45°C and 150r / min for 16h;

[0063] The above-mentioned seed medium comprises the following components by mass concentration:

[0064] Yeast extract powder 10g / L, peptone 10g / L, beef extract powder 5g / L, NaCl 10g / L, PH6.0;

[0065]...

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Abstract

The invention discloses a Bacillus velezensis strain and application thereof. The Fb strain is obtained by enrichment culture, primary screening, secondary screening and the like, belongs to the Bacillus velezensis and is gram-positive. The strain is characterized in that: the strain is rod-shaped, 0.4-0.6 microns * 0.9-4.0 microns, and is arranged singly or in pairs; and spores are nearly circular, near-end and cyst is expanded. Through detection, the Bacillus velezensis Fb can resist high temperature of 55 DEG C, the optimal growth temperature is 45 DEG C, and the optimal growth pH value is3.0-7.6. The strain can efficiently degrade tea residues, improve the protein, amino acid and the like of the tea residues and reduce the moisture, cellulose and the like of the tea residues, and thefermented tea residues can be used as a feed raw material; and the strain can realize smokeless, odorless and sewage-free discharge and can realize the cyclic utilization of organic wastes so as to reduce the pollution of the tea residues to the environment, and is a technology which accords with the environmental protection.

Description

technical field [0001] The invention relates to the field of industrial microbes, and more specifically relates to a strain of Bacillus velesi and its application. Background technique [0002] Bacillus Velez ( Bacillus velezensis ) is a new species of Bacillus, because of its inhibitory effect on pathogenic bacteria as a biological control regulator in agricultural production, the bacteria can produce secondary metabolites with broad-spectrum antibacterial activity, which is used to increase crop yield, maintain The biopharmaceutical of choice for ecoenvironmental and agroecosystems. There is no research report on the application of Bacillus veilei in the degradation of industrial and agricultural by-products at home and abroad. Tea dregs are the by-products of tea leaves used to produce instant tea or extract tea polyphenols. As one of the major tea producing countries in the world, my country produces hundreds of thousands of tons of tea dregs every year. Analysis sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/12C12R1/07
CPCA23K10/12C12R2001/07C12N1/205
Inventor 缪伏荣陈鑫珠刘景董志岩李忠荣
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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