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Biomacromolecule cracking device and detection system thereof

A biomacromolecule and cracking device technology, applied in the field of biomacromolecule cracking devices and detection systems thereof, can solve problems such as the inability to achieve uniform size and controllability of products, and achieve additional use costs, convenient operation methods, and high degradation efficiency. Effect

Pending Publication Date: 2020-06-30
DALIAN POLYTECHNIC UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The few methods used in polysaccharide structure analysis, such as acid degradation and enzymatic degradation, cannot achieve the ideal effect of uniform product size and controllable

Method used

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  • Biomacromolecule cracking device and detection system thereof
  • Biomacromolecule cracking device and detection system thereof
  • Biomacromolecule cracking device and detection system thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] The structure of the biomacromolecule detection system is as follows: figure 1 shown. The purified abalone polysaccharide was used as material for controlled fragmentation and ionization.

[0080] Abalone polysaccharide is a kind of marine animal polysaccharide, and its sugar content reaches more than 90% in the purified material after measurement. The abalone polysaccharide was dissolved in pure water to prepare a 5% aqueous solution, and 2 mL was injected into the quartz medium ionization chamber from the injection port. After that, set the input voltage 60, 70, 80, 90V for 5 minutes and input current 1A for the upper and lower electrodes; or set the input voltage for 60V and input current 1A for 3, 5 and 7 minutes for the upper and lower electrodes . After the ionization stops, a small amount of water can be replenished from the replenishment pipe to maintain the original volume. Then, the retractable heating electrode extends into the quartz medium ionization ch...

Embodiment 2

[0087] The structure of the biomacromolecule detection system is as follows: figure 1 shown. The purified sea cucumber glycoprotein complex was used as the material for controlled fragmentation and ionization.

[0088] Sea cucumber glycoprotein complex is a marine animal glycoprotein, mainly composed of polysaccharides and proteins. The sea cucumber glycoprotein complex was dissolved in pure water to prepare aqueous solutions with concentrations of 1mg / mL, 3mg / mL and 5mg / mL, and then the device was used for plasma degradation. The injection volume is 2mL, and it is injected into the quartz medium ionization chamber from the injection port. After that, the upper and lower electrodes are electrified and ionized for 6 minutes, and the input voltage is set to 60V, and the input current is 1A. After the ionization stops, the retractable heating electrode extends into the quartz medium ionization chamber to contact the sample solution, and heats at 95°C for 2 minutes, then stops. ...

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Abstract

The invention relates to a biomacromolecule cracking device and a detection system thereof. The biomacromolecule cracking device comprises: an ionization chamber which includesan inner chamber, a sample inlet and a sample outlet, wherein the inner chamber is used for accommodating a sample, and the sample inlet and the sample outlet are communicated with the inner chamber; a low-temperature discharge unit which comprises an upper electrode and a lower electrode, the ionization chamber is arranged between the upper electrode and the lower electrode, and the upper electrode and the lower electrode are opposite to the inner chamber; a heating electrode which is used for heating the sample in the inner chamber; and an ultrasonic electrode wich is used for carrying out ultrasonic treatment on the sample in the inner chamber. The biomacromolecule cracking device provided by the invention can degrade water-soluble biomacromolecules into micromolecule fragment products with uniform products and controllable sizes.

Description

technical field [0001] The present application generally relates to the field of plasma ionization, and specifically relates to a biomacromolecule cracking device and a detection system thereof. Background technique [0002] The study of the molecular structure of biological macromolecules is an important research content in the fields of basic research such as biochemistry and biophysics. At present, a complete research strategy has been accumulated in the structural research of macromolecular proteins and macromolecular nucleic acids, but there is still no effective and reliable research plan for the study of macromolecular polysaccharides. The key limiting factor is the inability to effectively decompose macromolecular polysaccharides into small molecular fragments with uniform products and controllable sizes. The research strategy of comprehensive protein and nucleic acid can be concluded that the consistent strategy in the structure analysis of these macromolecules is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72
Inventor 杨静峰孙景赫王艳杰
Owner DALIAN POLYTECHNIC UNIVERSITY
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