Preparation method of beta-nicotinamide mononucleotide
A single nucleotide and nicotinamide technology, applied in the field of biomedicine, can solve problems such as product safety hazards, complex processes, and unstable processes
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[0030] Example 1
[0031] Step A: Preparation of Nicotinamide Mononucleotide Sodium Salt:
[0032]
[0033] In a 2000 mL three-necked flask, nicotinamide ribose chloride (100 g, 0.344 mol) was added, and 1000 mL of pH=9 aqueous sodium hydroxide solution was added. After the temperature was controlled to 30° C. to stabilize, sodium trimetaphosphate (STMP) (110.48 g, 0.361 mol) was added to react for 4 hours. The reaction progress was monitored by high performance liquid chromatography. HPLC showed that 85% of the starting material was consumed after 4 hours, and the content of the product nicotinamide mononucleotide accounted for 83%.
[0034] Step B: Preparation of Nicotinamide Mononucleotide
[0035]
[0036] The reaction system was cooled to 10° C., 1M hydrochloric acid was added, the pH value of the reaction system was adjusted to 3.5, and the sodium salt of nicotinamide mononucleotide was acidified to become the inner salt form with structure I.
[0037] Step C: N...
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[0044] Example 2
[0045] Step A: Preparation of Nicotinamide Mononucleotide Potassium Salt:
[0046]
[0047] In a 2000 mL three-necked flask, nicotinamide ribose chloride (100 g, 0.344 mol) was added, and 1000 mL of pH=9 aqueous sodium hydroxide solution was added. After the temperature was controlled to 30° C. to stabilize, sodium trimetaphosphate (STMP) (110.48 g, 0.361 mol) was added to react for 4 hours. The reaction progress was monitored by high performance liquid chromatography. HPLC showed that 79% of the starting material was consumed after 4 hours, and the content of the product nicotinamide mononucleotide accounted for 76%.
[0048] Step B: Preparation of Nicotinamide Mononucleotide
[0049]
[0050] The reaction system was cooled to 10° C., 1ML phosphoric acid was added, the pH value of the reaction system was adjusted to 3.5, and the sodium salt of nicotinamide mononucleotide was acidified to become the inner salt form with structure I.
[0051] Step C: N...
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