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Grass carp excellent line molecule-assisted breeding and breeding effect verification method

A molecular-assisted, grass carp technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems that do not involve grass carp resistance to viruses, salt tolerance, hypoxia tolerance, development limitations of transgenic grass carp, low transgenic recognition rate, etc. To achieve the effect of shortening the breeding period, grass carp breeding and verification methods are feasible, and high social recognition

Active Publication Date: 2015-04-08
SHANGHAI OCEAN UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the approval rate of the public on genetic modification is generally low, and the development of genetically modified grass carp is subject to certain restrictions
[0004] Chinese patent document 200910197874.X discloses a method for the construction of a cultured grass carp family and a method for breeding improved species. This method can breed new varieties of grass carp with high quality, high yield, and stable yield, but it needs to separately treat the summer flowers, It takes a long time to optimize five stages of 1st, 2nd, 3rd, and 4th-year-old fish, and this method does not involve the selection of excellent strains of grass carp, such as anti-virus, salt-tolerant, and low-oxygen tolerance.

Method used

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  • Grass carp excellent line molecule-assisted breeding and breeding effect verification method
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  • Grass carp excellent line molecule-assisted breeding and breeding effect verification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Breeding and effect verification of excellent strains of grass carp with growth advantage and resistance to Aeromonas hydrophila

[0023] (1) Collect 600 pairs of grass carp in the Pearl River system, and select 250 pairs of male and female grass carp that are well-proportioned, lively and healthy, with a body weight 15-20% higher than the average body weight, and reach sexual maturity as parents to be selected; use the PIT marker (Shanghai Zhuhai Instrument Equipment Co., Ltd., HPT8) numbered each parent to be selected, and clipped the pectoral fins of each fish, used 12 pairs of microsatellites (Table 1) to carry out STR typing on the clipped pectoral fins, and used Cervus for the typing results 3.0 software analyzes the genetic diversity of these parents to be selected (see literature, Ren Kun et al., microsatellite paternity identification of grass carp, Journal of Southern Agricultural Sciences, 2013, 44 (8): 1367-1371); select 100 pairs with higher Genet...

Embodiment 2

[0033] Example 2 Breeding and effect verification of excellent strains of grass carp with growth advantage and salt tolerance

[0034] (1) Collect 600 pairs of grass carp from the Pearl River water system, and select 300 pairs of male and female grass carp that are well-proportioned, lively and healthy, with a body weight 15-20% higher than the average body weight, and reach sexual maturity as parents to be selected; use the PIT marker (Shanghai Zhuhai Instrument Equipment Co., Ltd., HPT8) numbered each parent to be selected, and clipped the pectoral fins of each fish, used 12 pairs of microsatellites (Table 1) to carry out STR typing on the clipped pectoral fins, and used Cervus for the typing results 3.0 software analyzes the genetic diversity of these parents to be selected (see literature, Ren Kun et al., microsatellite paternity identification of grass carp, Journal of Southern Agricultural Sciences, 2013, 44(8): 1367-1371), and 120 pairs with higher Genetically diverse a...

Embodiment 3

[0039] Example 3 Breeding and effect verification of excellent strains of grass carp with growth advantage and high temperature resistance

[0040] (1) Collect 600 pairs of grass carp from the Pearl River water system, and select 280 pairs of male and female grass carp that are well-proportioned, lively and healthy, with a body weight 15-20% higher than the average body weight, and reach sexual maturity as parents to be selected; use the PIT marker (Shanghai Zhuhai Instrument Equipment Co., Ltd., HPT8) numbered each parent to be selected, and clipped the pectoral fins of each fish, used 12 pairs of microsatellites (Table 1) to carry out STR typing on the clipped pectoral fins, and used Cervus for the typing results 3.0 software analyzes the genetic diversity of these parents to be selected (see literature, Ren Kun et al., microsatellite paternity identification of grass carp, Journal of Southern Agricultural Sciences, 2013, 44(8): 1367-1371), and 80 pairs with higher Genetical...

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Abstract

The invention relates to a grass carp excellent line molecule-assisted breeding and breeding effect verification method. The method comprises the following steps: grass carps with good morphological characteristics and high genetic diversity are selected as parents, and are marked as G1; hormones are injected for maturing, and artificial insemination is carried out, such that fertilized eggs are obtained; 1-year-old breeding fish cultivation is carried out; in December of the same year, individuals with growth advantages in G1 are selected and are subjected to resistance tests; corresponding parents G1A of survived individuals are found with a grass carp paternity test technology; the G1A and second year offspring g1A are verified, such that an excellent line is determined. The grass carp excellent line breeding and verification method provided by the invention has a short period. With the method, excellent grass carp lines with specific resistances can be bred according to corresponding different cultivation environment needs.

Description

【Technical field】 [0001] The invention relates to a method for breeding an excellent variety of grass carp and verifying its effect, in particular to a method for molecular-assisted breeding of an excellent grass carp strain and verifying the effect of the breeding. 【Background technique】 [0002] Grass carp is one of the four major fish species in my country, and has a history of nearly 2,000 years of farming in China. In the early days, grass carp fish fry were mainly caught from natural water bodies, and relying on the weather has always restricted the development of grass carp farming industry; after the breakthrough of artificial breeding technology of grass carp, large-scale grass carp farming has appeared in my country; at present, the output of grass carp has been maintained at the level of freshwater aquaculture for many years. first. However, the grass carp breeding industry is still facing many problems. The most important problem is that the germplasm resources o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A01K61/10A01K67/027
CPCA01K61/00Y02A40/81
Inventor 李家乐张猛沈玉帮傅建军孙俊龙徐晓雁党云飞
Owner SHANGHAI OCEAN UNIV
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