A method for molecularly assisted breeding of superior strains of grass carp and verification of breeding effect

Abstract

The invention relates to a grass carp excellent line molecule-assisted breeding and breeding effect verification method. The method comprises the following steps: grass carps with good morphological characteristics and high genetic diversity are selected as parents, and are marked as G1; hormones are injected for maturing, and artificial insemination is carried out, such that fertilized eggs are obtained; 1-year-old breeding fish cultivation is carried out; in December of the same year, individuals with growth advantages in G1 are selected and are subjected to resistance tests; corresponding parents G1A of survived individuals are found with a grass carp paternity test technology; the G1A and second year offspring g1A are verified, such that an excellent line is determined. The grass carp excellent line breeding and verification method provided by the invention has a short period. With the method, excellent grass carp lines with specific resistances can be bred according to corresponding different cultivation environment needs.

Description

【Technical field】 [0001] The invention relates to a method for breeding an excellent variety of grass carp and verifying its effect, in particular to a method for molecular-assisted breeding of an excellent grass carp strain and verifying the effect of the breeding. 【Background technique】 [0002] Grass carp is one of the four major fish species in my country, and has a history of nearly 2,000 years of farming in China. In the early days, grass carp fish fry were mainly caught from natural water bodies, and relying on the weather has always restricted the development of grass carp farming industry; after the breakthrough of artificial breeding technology of grass carp, large-scale grass carp farming has appeared in my country; at present, the output of grass carp has been maintained at the level of freshwater aquaculture for many years. The first one. However, the grass carp breeding industry is still facing many problems. The most important problem is that the germplasm res...

AI Technical Summary

Problems solved by technology

However, the approval rate of the public on genetic modification is generally low, and the development of genetically modified grass carp is subject to certain restrictions

[0004] Chinese patent document 200910197874.X discloses a method for the construction of a cultured grass carp family and a method for breeding improved species. This method can breed new varieties of grass carp with high quality, high yield, and stable yield, but it needs to separately treat the summer flowers, It takes a long time to optimize five stages of 1st, 2nd, 3rd, and 4th-year-old fish, and this method does not involve the selection of excellent strains of grass carp, such as anti-virus, salt-tolerant, and low-oxygen tolerance.

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