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Animal immune lymphocyte separation liquid and preparation method thereof

A technology of lymphocytes and separation liquid, which is applied in the field of animal lymphocyte separation liquid and its preparation, and can solve problems such as application limitations of hydroxyethyl starch

Inactive Publication Date: 2020-07-07
CHENGDU ZHIA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The clinical application of hydroxyethyl starch is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A liquid for separating animal lymphocytes, characterized in that it comprises the following components in mass percentage: 5% iotalaglumine, 3% hydroxypropyl-β-cyclodextrin, 1,3:2,4-di (Benzylidene)-D-sorbitol 0.2%, ammonium glycyrrhizinate 0.1%, 1-allyl-3-methylimidazole chloride / maleimide-tri(ethylene glycol)-propionic acid / Polyethylene glycol monoallyl ether copolymer 0.5%, polyvinylpyrrolidone 0.8%, and the balance is deionized water without pyrogen.

[0024] The preparation method of described 1-allyl-3-methylimidazole chloride / maleimide-three (ethylene glycol)-propionic acid / polyethylene glycol monoallyl ether copolymer comprises as follows Steps: 10 g of 1-allyl-3-methylimidazole chloride, 10 g of maleimide-tris(ethylene glycol)-propionic acid, 20 g of polyethylene glycol monoallyl ether, and azobis 0.3 g of isobutyronitrile was dissolved in 150 g of dimethyl sulfoxide, stirred and reacted at 65° C. for 4 hours in a nitrogen atmosphere, and the solvent was rem...

Embodiment 2

[0027] An animal lymphocyte separation liquid is characterized in that it includes the following components in mass percentage: 6% iotalaglumine, 3.5% hydroxypropyl-β-cyclodextrin, 1,3:2,4-di (Benzylidene)-D-sorbitol 0.4%, ammonium glycyrrhizinate 0.2%, 1-allyl-3-methylimidazole chloride / maleimide-tri(ethylene glycol)-propionic acid / Polyethylene glycol monoallyl ether copolymer 0.7%, polyvinyl alcohol 0.9%, and the balance is deionized water without pyrogen.

[0028] The preparation method of described 1-allyl-3-methylimidazole chloride / maleimide-three (ethylene glycol)-propionic acid / polyethylene glycol monoallyl ether copolymer comprises as follows Steps: 10 g of 1-allyl-3-methylimidazole chloride, 10 g of maleimide-tris(ethylene glycol)-propionic acid, 23 g of polyethylene glycol monoallyl ether, and azobis Dissolve 0.35 g of isoheptanonitrile in 170 g of N,N-dimethylformamide, stir and react at 68° C. for 4.5 hours in a helium atmosphere, and then remove the solvent by r...

Embodiment 3

[0031] An animal lymphocyte separation liquid is characterized in that it includes the following components in mass percentage: 8% iotalaglumine, 4% hydroxypropyl-β-cyclodextrin, 1,3:2,4-di (Benzylidene)-D-sorbitol 0.7%, ammonium glycyrrhizinate 0.35%, 1-allyl-3-methylimidazole chloride / maleimide-tri(ethylene glycol)-propionic acid / Polyethylene glycol monoallyl ether copolymer 1%, polyacryl alcohol 1.1%, and the balance is deionized water without pyrogen.

[0032]The preparation method of described 1-allyl-3-methylimidazole chloride / maleimide-three (ethylene glycol)-propionic acid / polyethylene glycol monoallyl ether copolymer comprises as follows Steps: 10 g of 1-allyl-3-methylimidazole chloride, 10 g of maleimide-tris(ethylene glycol)-propionic acid, 25 g of polyethylene glycol monoallyl ether, and azobis Dissolve 0.4 g of isobutyronitrile in 175 g of N-methylpyrrolidone, stir and react at 70°C for 5 hours in a neon atmosphere, and then remove the solvent by rotary evaporat...

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Abstract

The invention discloses an animal lymphocyte separation liquid. The animal lymphocyte separation liquid is characterized by being prepared from the following components in percentage by mass: 5-10% ofIotalamate Meglumine, 3-5% of hydroxypropyl-beta-cyclodextrin, 0.2-1% of 1,3:2,4-bis (benzylidene)-D-sorbitol, 0.1-0.5% of ammonium glycyrrhizinate, 0.5-1.5% of 1-allyl-3-methyl imidazole chloride / Maleimide-Tri(ethylene glycol)-Propionic acid / polyethylene glycol monoallyl ether copolymer, 0.8-1.5% of a nonglycosylated hydrophilic polymer and the balance of deionized water without a heat source. The invention further discloses a preparation method of the animal lymphocyte separation liquid. According to the animal lymphocyte separation liquid, the safety, the separation efficiency and purity can be ensure, cytotoxicity is not existed, the endotoxin content is low, separated lymphocytes are good in condition and high in vitality, in addition to being used in scientific research, the animallymphocyte separation liquid can be widely used in clinical diagnosis, and the animal lymphocyte separation liquid has good market prospects.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an animal lymphocyte separation liquid and a preparation method thereof. Background technique [0002] As a kind of effector cells, lymphocytes widely exist in human peripheral blood, bone marrow or human umbilical cord blood. The acquisition of high-purity lymphocytes is of great significance for the cytological diagnosis of diseases, component blood transfusion, and the preparation of therapeutic cell products. In clinical medical testing, the examination of specific cell functions and biological characteristics often involves cell separation techniques. Isolation of mononuclear cells from peripheral blood and various body fluids (pleural effusion, ascites, pericardial effusion, etc.) is performed in vitro. As an important pretreatment step in cellular immunology research and cytological experiments, the quality and quantity of isolated target cells is an important link to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078
CPCC12N5/0634C12N2509/00
Inventor 向兴力
Owner CHENGDU ZHIA TECH CO LTD
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