Brevibacillus laterosporus and application thereof

A technology of Brevibacillus brevis and short spore lateral spore, applied in the field of microorganisms, can solve the problems of weakened self-purification ability, high pressure on aquaculture, single cultured species, etc., so as to overcome the abuse of antibiotics, reduce fish and shrimp diseases, and overcome the frequent occurrence of epidemic diseases. Effect

Active Publication Date: 2020-07-10
武汉合缘绿色生物股份有限公司
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Problems solved by technology

However, in order to pursue economic benefits, on the one hand, the breeding density and frequency are too high, and the breeding species is single, which leads to the destruction of the balance of the natural ecological system in the breeding water and serious self-pollution; The large accumulation of excreta leads to the proliferation of harmful microorganisms, which deteriorates

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  • Brevibacillus laterosporus and application thereof
  • Brevibacillus laterosporus and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, screening and identification of Brevibacillus spp.

[0018] S1. Experimental materials

[0019] The water samples come from the fish and shrimp breeding base of Wuhan Heyuan Green Biological Company, and are collected from the breeding ponds with serious water pollution and high incidence of fish and shrimp.

[0020] Beef extract peptone liquid medium: beef extract 3g, peptone 8g, sodium chloride 4g, dilute to 1000mL with distilled water, pH7.5; beef extract peptone solid medium is beef extract peptone liquid medium, add 18g / L agar. The medium was sterilized with high pressure steam at 121°C for 20 min before use.

[0021] The pathogenic bacteria are represented by Vibrio parahaemolyticus, Pseudomonas punctatus, Pseudomonas albicans and Escherichia coli, all donated by the Fisheries College of Huazhong Agricultural University.

[0022] S2. Primary screening of strains

[0023] The water samples were taken and spread on the beef extract peptone solid med...

Embodiment 2

[0034] Embodiment 2, the preparation of microbial bacterial agent

[0035] Inoculate a single colony of Brevibacillus lateralosporus HY30 of the present invention activated on beef extract peptone solid medium into beef extract peptone liquid medium, and vibrate at 30°C and 180r / min until mid-logarithmic growth, and obtain seeds liquid.

[0036] The present invention selects glucose, sucrose, starch and cornstarch as carbon source alternatives; selects urea, beef extract, industrial peptone and yeast extract as nitrogen source alternatives, and it is confirmed by single factor optimization and orthogonal experiments that the Brevispora lateralis The optimum fermentation medium for bacillus HY30 is: glucose 20.0g / L, yeast extract 10.0g / L, MnSO 4 0.2g / L, CaCl 2 0.3g / L, KH 2 PO 40.1g / L, MgSO 4 0.05g / L.

[0037] Use the same formulation of fermentation medium in a 5L fermenter, and the fermentation conditions are: 3L of sterilized fermentation medium input, initial pH val...

Embodiment 3

[0038] Embodiment 3, the application of microbial bacterial agent in freshwater aquaculture

[0039] S1. Detection of water quality improvement effect

[0040] Get the higher fish and shrimp culture pond waste water of organic matter content, after mixing, be sub-packed in 8L plastic bucket, every bucket 4L, test group adds the microbial bacterial agent described in embodiment 2 and dilutes 10 8 Add 40mL of sterilized water to the control group, let it stand still, and measure the pH value, nitrite and ammonia nitrogen of the water samples in the control group and the test group at 0h, 4h, 8h, 12h, 25h, 30h, 35h, and 45h. Content value, every group of tests was repeated 3 times, and the results are shown in Table 3, and the data in Table 3 are the average value of three repeated tests.

[0041] Table 3 Water quality improvement effect test result table

[0042]

[0043] As shown in Table 3, the pH value of the water samples in the control group was relatively high and rel...

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Abstract

The invention provides Brevibacillus laterosporus and application thereof. The strain is called after Brevibacillus laterosporus HY30 and has an accession number of CCTCC No: M20191101. The strain provided by the invention can be used for degrading ammonia nitrogen and nitrite nitrogen in water and has a relatively high inhibitory effect on freshwater aquaculture pathogens such as vibrio parahaemolyticus, Pseudomonas hydrophila, Pseudomonas dermoalba and Escherichia coli, so that the strain provided by the invention can be developed and applied to microbial agents for water purification and bacteriostasis of freshwater aquaculture, the occurrence of diseases of fish-shrimp culture is effectively prevented, and the quality and yield of fish-shrimp products are increased.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Brevibacillus sporogenes and its application. Background technique [0002] In recent years, with the continuous development of our country and the continuous increase of population, people's demand for food has gradually increased. Aquatic products have won people's general favor for their rich nutrition and delicious taste, and have become one of the indispensable foods on the table. one. What followed was the vigorous development of the aquaculture industry, which greatly enriched people's material life. However, in order to pursue economic benefits, on the one hand, the breeding density and frequency are too high, and the breeding species is single, which leads to the destruction of the balance of the natural ecological system in the breeding water and serious self-pollution; The large accumulation of excreta leads to the proliferation of harmful microorg...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01K61/13C12R1/13
CPCC12N1/20A01K61/13C12R2001/13C12N1/205Y02A40/81
Inventor 莫晓静詹巧春佀国涵张业成袁庆丰徐维烈
Owner 武汉合缘绿色生物股份有限公司
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