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Molecular targets of cervical squamous cell carcinoma and their application in diagnosis and treatment

A technology of cervical squamous cell carcinoma and reagent, applied in the field of biomedicine, can solve the problems of high metastasis and recurrence rate and unsatisfactory effect, etc.

Active Publication Date: 2020-12-22
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When cervical cancer further develops to the advanced stage, the effect of surgical resection of cancerous tissue combined with concurrent chemotherapy and radiotherapy is not very satisfactory, and the metastasis and recurrence rates are still high

Method used

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  • Molecular targets of cervical squamous cell carcinoma and their application in diagnosis and treatment
  • Molecular targets of cervical squamous cell carcinoma and their application in diagnosis and treatment
  • Molecular targets of cervical squamous cell carcinoma and their application in diagnosis and treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1 QPCR detects the expression of RNF167 gene

[0063] 1. Sample collection

[0064] 33 cervical squamous cell carcinoma tissues and corresponding paracancerous tissues were collected. All cases did not receive any immunosuppressant treatment, radiotherapy and chemotherapy before operation.

[0065] 2. RNA extraction

[0066] Tissue total RNA was extracted using TRIZOL method.

[0067] 1) Mince the tissue with scissors, add 1ml Trizol, shake on the shaker for 1min; place at room temperature for 10min to completely decompose the ribosomes.

[0068] 2) Add 200 μl of chloroform (chloroform), close the cap tightly, shake vigorously for 15 seconds, and let stand at room temperature for 10 minutes.

[0069] 3) Centrifuge at 11000 rpm for 15 minutes at 4°C.

[0070] 4) Transfer the water sample layer to a new centrifuge tube, add 500 μl of isopropanol; after inverting to mix well, let stand at room temperature for 10 minutes.

[0071] 5) Centrifuge at 11000 rpm ...

Embodiment 2

[0106] Example 2 Effect of RNF167 on cervical squamous cell carcinoma cells

[0107] 1. Construction of overexpression vector

[0108] According to the RNF167 gene sequence information in NCBI, specific primers were designed and amplified to obtain the corresponding genes. After the gene fragments were recovered by gel, they were connected with the expression vector pGL3 basic and transformed into DH5α competent cells. Positive clones were picked for PCR and sequencing. After identification, the pGL3 basic-RNF167 recombinant plasmid was obtained.

[0109] 2. Cell culture

[0110] Cervical squamous cell carcinoma cells (Hela) were cultured in RPIM-1640 medium containing 10% fetal bovine serum and 1% P / S at 37°C, 5% CO 2 When the cells grew to 80%-90%, they were routinely digested and passaged with 0.25% EDTA-containing trypsin.

[0111] 3. Transfection

[0112] The experiment was divided into 3 groups, namely the control group (Hela), the transfection empty group (transfect...

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Abstract

The present invention discloses the molecular target of cervical squamous cell carcinoma and its application in diagnosis and treatment. Through QPCR detection, it is first found that the expression of RNF167 is down-regulated in patients with cervical squamous cell carcinoma, and RNF167 is used as a variable with high sensitivity and specificity , indicating that RNF167 can be used as a molecular marker in the diagnosis of cervical squamous cell carcinoma. By overexpressing RNF167, it was found that the proliferation and invasion ability of cervical cancer cells were significantly affected, indicating that RNF167 can be used as a molecular target in the treatment of cervical squamous cell carcinoma.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a molecular target of cervical squamous cell carcinoma and its application in diagnosis and treatment. Background technique [0002] Cervical cancer (CC) is the most common malignant tumor of the female reproductive system (Chen, W., et al., Cancer statistics in China, 2015. CA Cancer J Clin, 2016.66 (2): p115-32.), its The incidence rate ranks second among gynecological malignancies. According to the World Health Organization (WHO), about 53,000 new cases of cervical cancer are added each year, and as many as 25,000 patients die from cervical cancer (Zhou Qi. Cervical Cancer Diagnosis and Treatment Guidelines (Fourth Edition). Chinese Journal of Practical Gynecology and Obstetrics, 2018, 34(6):613-622.). Due to the popularization of cervical cancer screening in western developed countries, the incidence of cervical cancer has slowly declined, while the situation in developing countries ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/574A61K45/00A61K48/00A61K38/17A61P35/00
CPCA61K38/177A61K45/00A61K48/005A61P35/00C12Q1/6886C12Q2600/136C12Q2600/158G01N33/57411G01N33/57484G01N2333/705
Inventor 彭雪
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN