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Method for producing fucosterol drug intermediates and algal polysaccharides

A technology of fucosterol and algal polysaccharide, applied in the field of biomedicine, can solve problems such as low content, and achieve the effects of high production rate, reduced energy consumption, and improved leaching rate

Pending Publication Date: 2020-07-14
HANGZHOU BROTHER BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sargassum contains sterols and polysaccharides, but the content is low. How to improve the content of useful substances by optimizing the culture conditions of Sargassum is a technical problem that we need to solve

Method used

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  • Method for producing fucosterol drug intermediates and algal polysaccharides
  • Method for producing fucosterol drug intermediates and algal polysaccharides
  • Method for producing fucosterol drug intermediates and algal polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A process for improving the efficiency of fucosterol synthesis by sargassum, comprising the steps of:

[0037] The Sargassum used in the experiment comes from the coast of Ningbo, Zhejiang Province. Mature male and female algae are collected and cultivated artificially until the seedlings are about 1 cm long. Select healthy sargassum seedlings and inoculate them into a small reaction tank containing sargassum culture solution. The inoculation density is 80 plants / ㎡, the depth of the culture solution in the reaction tank is 30cm, and the culture solution is replaced every 4 days. 24 days; culture conditions: light intensity 3000lux, temperature 22°C, light-to-dark ratio 12:12, air flow 15ml / min·L; Harvest Sargassum algae, dry naturally for 5 days in a ventilated place, and then place in Pulverize with a pulverizer and pass through a 50-mesh sieve to obtain the sargassum algae powder.

[0038] The sargassum culture solution is obtained by improving the formula of the f / 2 m...

Embodiment 2

[0040] A process for improving the efficiency of fucosterol synthesis by sargassum, comprising the steps of:

[0041] The Sargassum used in the experiment comes from the coast of Ningbo, Zhejiang Province. Mature male and female algae are collected and cultivated artificially until the seedlings are about 2cm in length. Select healthy Sargassum seedlings and inoculate them into a small reaction tank containing Sargassum culture solution. The inoculation density is 70 plants / ㎡, the depth of the culture solution in the reaction tank is 40cm, and the culture solution is replaced every 4 days. for 24 days; the culture conditions are: light intensity 3000lux, temperature 23°C, light-dark ratio 14:10, air flow 20ml / min·L; Harvest Sargassum algae, dry naturally for 5 days in a ventilated place, and then place in Pulverize with a pulverizer and pass through a 50-mesh sieve to obtain the sargassum algae powder.

[0042] The sargassum culture solution is obtained by improving the formu...

Embodiment 3

[0047] Effects of various culture factors on the synthesis of fucosterol in Sargassum spp.

[0048] On the basis of Comparative Example 1, the effects of different light intensities on the synthesis of fucosterol by Sargassum sargassum were verified.

[0049] Set the light intensity as: 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000, 10000, the unit is lux.

[0050] Crude extraction and detection method of fucosterol:

[0051] Add sargassum algae powder to the alcohol extraction tank, the ratio of algae powder to 85% ethanol is 1g:5ml, heat up to 50°C, under the condition of heat preservation, ultrasonic (power 300W, frequency 25kHZ) assists alcohol extraction for 30min, Stop the ultrasonic treatment, and continue the heat preservation alcohol extraction for 9 hours; then vacuum suction filtration, collect the filter residue and filtrate, place the filtrate at 65°C and concentrate under reduced pressure to recover ethanol until it is concentrated to one-fifth of the original ...

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Abstract

The invention belongs to the technical field of biology, and discloses a method for producing fucosterol drug intermediates and algal polysaccharides, which comprises the following steps: step 1) culturing sargassum thunbergii, step 2) preparing algal powder, step 3) preparing a fucosterol crude product, step 4) purifying fucosterol, and step 5) separating algal polysaccharides. According to the method disclosed by the invention, the synthesis efficiency of the fucosterol is improved, algal polysaccharides are obtained while the fucosterol is separated and extracted, and the co-production of two effective substances is realized.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for producing fucosterol drug intermediates and algal polysaccharides. Background technique [0002] Fucosterol widely exists in brown algae and kelp, white powder, soluble in non-polar organic solvents, insoluble in ethanol, acetone, insoluble in water. Fucosterol has very important physiological functions, such as maintaining the stability of the biological internal environment, controlling the metabolism of glycogen and minerals, regulating stress response, preventing and treating cancer, and significantly reducing blood cholesterol. Therefore, the role of fucosterol has attracted the attention of scientists from various countries. At the same time, fucosterol can be used as an intermediate for the production of important pharmaceutical steroid compounds, such as estrogen, androgen, hydrocortisone, etc., which can regulate human growth, reproduction, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00C08B37/00A01G33/00
CPCC07J9/00C08B37/0003A01G33/00Y02A40/80
Inventor 刘加林霍萧勇刘加海方朝杰张渊源
Owner HANGZHOU BROTHER BIO TECH CO LTD
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