A fully enclosed cell substrate tensile and flow shear composite loading experimental system

Abstract

The embodiment of the present invention relates to a fully-sealed cell substrate tensile and flow-shear composite loading experimental system, which can realize tensile and flow-shear composite loading on the substrate of a conventionally cultured number of cells, and can quantitatively control loading parameters and automatically operate . Use the motor to drive the driving mechanism to pull the pull rods at both ends, and set the stretching deformation ratio, stretching speed, and reciprocating stretching frequency through programming to achieve quantitatively controlled static or dynamic stretching. The culture medium is driven by a peristaltic pump, and quantitatively controlled flow shear can be achieved by setting the flow rate of the peristaltic pump. The combination of different composite loading methods can be carried out by setting the action time of base tension and flow shear respectively by programming.

Description

technical field [0001] The embodiments of the present invention relate to the field of biotechnology, and in particular to a fully enclosed cell substrate tensile and flow shear composite loading experiment system. Background technique [0002] When conducting cell biomechanical experimental research, special experimental devices are often required to perform real-time static or dynamic mechanical loading on cultured cells, and it is necessary to standardize and quantify the mechanical microenvironment of cell growth. Applying substrate tension or fluid shear to cells are two common ways of mechanically loading cells. At present, there are commercial substrate tensile loading devices and flow chamber flow shear loading devices. [0003] In order to simulate the complex mechanical environment in the body, it is a further experimental research requirement to perform compound loading of cells under various mechanical conditions, such as compound loading experiments of substrat...

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Problems solved by technology

However, its culture area is limited, because it is a non-planar substrate, which is not conducive to uniform cell seeding and microscopic observation.

There are also devices that use microchannels made of pulled PDMS silica gel to achieve composite loading, but microchannel devices cannot meet the needs of a large number of cell cultures

Although silica gel has excellent biocompatibility, the following problems still need to be solved when using larger silica gel elastic culture containers: the driving pressure when perfusing the culture solution can cause the silica gel culture container to expand and deform, and the cross section of the flow channel will change accordingly, making the flow shear Cut level is difficult to control stably

At present, there is no experimental device for cell substrate tensile and fluid shear composite loading that can comprehensively solve the above problems.

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