Construction method of radioactive cell damage model

Abstract

The invention discloses a construction method of a radioactive cell damage model. The method comprises the following steps: extracting bone marrow mesenchymal stem cells from bone marrow of femur andtibia of a young SD rat, and culturing and transferring the cells to a third generation; identifying the third generation of bone marrow mesenchymal stem cells; irradiating the bone marrow mesenchymalstem cells with gamma rays of a cobalt 60 radioactive source, wherein the irradiation dose gradient is 2,4,6 and 8 Gy, and the gamma ray dose rate is controlled at 2.538 Gy / min; carrying out integralcalculation on the boundary effect in the process of raising and lowering the source, so that the accumulated irradiation dose actually born by the bone marrow mesenchymal stem cells reaches the setdose; and according to proliferation capacity and apoptosis degree of the bone marrow mesenchymal stem cells, in combination with the screening detection of the living cells, screening and detecting the bone marrow mesenchymal stem cells according to select the bone marrow mesenchymal stem cells irradiated with specific gamma ray irradiation dose as a radioactive damage model. The method providesthe cell model with higher reliability and repeatability for the future research of ionizing radiation damage effect, radiation protection and related diseases of organisms.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to the establishment of an in vitro radiation model of bone marrow mesenchymal stem cells, in particular to a method for building a cobalt-60-based rat bone marrow mesenchymal stem cell model. Background technique [0002] Radiation therapy is a local treatment method for various malignant tumors. It is involved in the treatment of about 70% of malignant tumors, and about 40% of them can be cured only by radiotherapy. Radiation achieves the therapeutic effect on tumors by producing ionizing radiation effects on human tissue cells, but because radiation is not specific to cells and tissues, it is often accompanied by a series of complications, causing serious harm to the quality of life of patients . The types of rays commonly used in radiotherapy mainly include α, β, and γ rays produced by radioactive isotopes, X-rays and various particle beams produced by various X-ray therapy ma...

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Problems solved by technology

[0006] Based on the research status of the establishment of radiation injury models of radiation cells, the current research on cell radiation injury and related diseases mainly has the following technical problems: (1) The source of radiation is not uniform, and the difference between X-ray and γ-ray ionizing radiation damage is not well understood Clear; (2) The radiation dose is not uniform; (3) The radiation target cells are not uniform, which is not conducive to the analysis and judgment of the radiation effect; (4) The radiation damage evaluation indicators of radioactive cells are not uniform and not objective; (5) The repeatability is poor

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